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pEIF3D-NtermDel-CtermDel-sgEIF3D

Plasmid

#222131

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

161 + 527 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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PiggyBac-mProser1-FLAG-IRES-Blast

Plasmid

#226173

Purpose

Expresses full length mouse PROSER1 with 2X C-terminal FLAG tags

Depositor

Kasper Rasmussen

Article

Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

Insert

Proline Serine Rich protein 1 (Proser1 Mouse)

Tags

2X FLAG

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-crCoV-Mutiplex_EF1a-BFP

Plasmid

#224788

Purpose

SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crCoV20, crCoV21, crCoV24

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6 and hU6-2xTetO

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-S528N-S529N-sgEIF3D

Plasmid

#222129

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528N, S529N

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-TAPP1(PHD)

Plasmid

#221757

Purpose

Stable expression of GFP-TAPP1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PLEKHA1 (PLEKHA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem TAPP1 (PHD) (amino acids 184-304)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-Btk1(PHD)-GFP

Plasmid

#221756

Purpose

Stable expression of Btk1(PHD)-GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Btk (BTK Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–177

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-MCHpr-DREADD (Gq)-mCherry

Plasmid

#221036

Purpose

Expresses Gq coupled DREADD hM3D under the rat pro-melanin-concentrating hormone promoter

Depositor

Yanhua Huang

Article

Wang et al Mol Psychiatry. 2021 Jul;26(7):3152-3168

Insert

DREADD Gq-mCherry

Use

AAV; Rat targeting

Expression

Mammalian

Promoter

Rat MCH

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_CBPdIDR7-GFP

Plasmid

#221928

Purpose

Dox-inducible expression of CBPdeltaIDR7-GFP in mammalian cells by retroviral transduction

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBPdeltaIDR7 (CREBBP Human)

Use

Retroviral

Tags

GFP

Mutation

insertion of CBP-GFP; deletion of IDR7 (aa 2109-2…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdAL

Plasmid

#221910

Purpose

Transient expression of GFP-tagged CBPdeltaAL in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBPdeltaAL (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of the AL (aa 1558-1607); insertion at t…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdCFID

Plasmid

#221909

Purpose

Transient expression of GFP-tagged CBPdeltaCFID in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBPdeltaCFID (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of the CBP-FUS interaction domain (CFID)…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdIDR7dAL

Plasmid

#221914

Purpose

Transient expression of GFP-tagged CBPdeltaIDR7deltaAL in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of IDR7 (aa 2109-2434); deletion of the …

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdIDR6dAL

Plasmid

#221912

Purpose

Transient expression of GFP-tagged CBPdeltaIDR6deltaAL in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of IDR6 (aa 1856-2090); deletion of the …

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_CBPdIDR6-GFP

Plasmid

#221927

Purpose

Dox-inducible expression of CBPdeltaIDR6-GFP in mammalian cells by retroviral transduction

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBPdeltaIDR6 (CREBBP Human)

Use

Retroviral

Tags

GFP

Mutation

insertion of CBP-GFP; deletion of IDR6 (aa 1856-2…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D197, 199, 330, 332N IRES GFP

Plasmid

#195703

Purpose

Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D197, 199, 330, 332N …

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M2

Plasmid

#204560

Purpose

Expresses GST-tagged UFBP1 M2 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

28-115aa

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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phAG-MCL-CDK5RAP3 W269A W294A W312A

Plasmid

#204572

Purpose

Expresses hAG-tagged CDK5RAP3 W269A W294A W312A in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CDK5RAP3 (AMY2A Human)

Expression

Mammalian

Mutation

W269A W294A W312A

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M1

Plasmid

#204559

Purpose

Expresses GST-tagged UFBP1 M1 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

28-314aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIH FRB-BFP

Plasmid

#202425

Purpose

TagBFP FKBP-rapamycin binding (FRB) dimerization domain

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

FKBP-rapamycin binding (FRB) dimerization domain

Use

Lentiviral

Tags

TagBFP

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-P66KP71Q-GB1_AD

Plasmid

#148516

Purpose

Bacterial Expression of DmThor_50-83-P66KP71Q

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-83-P66KP71Q (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmThor-del76-84_Z

Plasmid

#148190

Purpose

Bacterial Expression of DmThor-del76-84

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-del76-84 (Thor Fly)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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