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MSCV-mir20

Plasmid

#24709

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

mir-20 (MIR20A Mouse, Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pCHNFI-B2

Plasmid

#31405

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IB isoform 2 (Nfib Mouse)

Tags

HA

Expression

Mammalian

Mutation

Not the same as reference seq. See sequence for m…

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pHIE041 CAG mKate2 (TUPV 1)

Plasmid

#138721

Purpose

mMoClo TUPV, with CAG promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

CAG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_MCS_version2

Plasmid

#197961

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-vCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_MCS_version2

Plasmid

#197962

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pco-nCasphi-2

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pXS-mPlum

Plasmid

#117388

Purpose

pGEN-mcs with a modular mPlum expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

mPlum

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-10Myc

Plasmid

#179814

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xMyc

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-10FLAG

Plasmid

#179815

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xFLAG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJZC506

Plasmid

#62281

Purpose

COM-VP64 fusion

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

COM-VP64

Tags

VP64

Expression

Yeast

Promoter

pAdh

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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EYFP-Sec23A

Plasmid

#66611

Purpose

YFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Tags

EYFP

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pMKO.1 GFP B56 alpha shRNA 6-3

Plasmid

#10680

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 alpha shRNA (PPP2R5A Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pN-PURO-PTP

Plasmid

#51711

Purpose

contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Available Since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

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FRB-polySH35

Plasmid

#175234

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Hy_pUbi-p63e eGFP SV40

Plasmid

#132650

Purpose

Expresses eGFP mRNA from the Drosophila Ubi-p63e promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

Ubi-p63e

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE 608 : pHYG_GFP

Plasmid

#92089

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mCherry

Plasmid

#179789

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mCherry

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Vh-HA-2xFRB

Plasmid

#20224

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

One copy of FRB has a T2098L mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pPD535 VP16-ZF129

Plasmid

#138768

Purpose

Constitutive expression of VP16-ZF129 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF129

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pOGG009

Plasmid

#113983

Purpose

Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Gentamicin

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pPD303 VP64-ZF43

Plasmid

#138835

Purpose

Constitutive expression of VP64-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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