We narrowed to 10,236 results for: ada

1. pLenti Dest PuroR R1-R2

   Plasmid

   #84575

   Purpose

   Gateway destination vector expressing Puromycin resistance under a PGK promoter

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Gateway

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV10.KH

   Plasmid

   #63208

   Purpose

   pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5FRT/TO-UBXD7-Strep-HA

   Plasmid

   #113479

   Purpose

   Expression of human UBXD7 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBXD7 (UBXN7 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   A834C silent mutation, wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEX-HcRed-hLC3WT

   Plasmid

   #24991

   Depositor

   Isei Tanida

   Article

   Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

   Insert

   a wild type human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
   

   Tags

   HcRed

   Expression

   Mammalian

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pCMV10-Flag-Fbxl21

   Plasmid

   #48752

   Purpose

   Expression of FLAG-tagged Fbxl21

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   Fbxl21 (Fbxl21 Mouse)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pRvACT-mEGFP-NLS

   Plasmid

   #205001

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

   Plasmid

   #159813

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PITCh-MYO1C

   Plasmid

   #227305

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of MYO1C (MYO1C Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159850

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC_N_SOX2

    Plasmid

    #167794

    Purpose

    Expresses SOX2 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    SOX2 (SOX2 Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMHE-mCherry-mTrim21ΔRING-Box

    Plasmid

    #105523

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLENTI_RPS2

    Plasmid

    #127127

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS2 (RPS2 Human)
    

    Use

    Lentiviral

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe puro RalA Q75L

    Plasmid

    #19719

    Depositor

    Channing Der

    Article

    Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

    Insert

    V-ral simian leukemia viral oncogene homolog A (RALA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    GTPase deficient & constitutively active Gl…

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5FRT/TO-p37-Strep-HA

    Plasmid

    #113485

    Purpose

    Expression of human p37 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    p37 (UBXN2B Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pL6-H2B-iRFP720-puro

    Plasmid

    #189992

    Purpose

    Lentivirial plasmid for nuclear iRFP-720 expression with puromycin resistance

    Depositor

    Achim Kramer

    Article

    Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    Human histone H2B
    

    Use

    Lentiviral

    Tags

    iRFP720

    Expression

    Mammalian

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-C-Solo-mStayGold-PXN

    Plasmid

    #227316

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PXN Homology Arms flanking a mStayGold Tag (PXN Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHLmMBP-2

    Plasmid

    #72344

    Purpose

    Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP

    Expression

    Mammalian

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-2XFLAG,2XHA-CyclinF-puro

    Plasmid

    #32976

    Depositor

    Michele Pagano

    Article

    D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

    Insert

    CYCLIN F (CCNF Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pHR RPS27A-3xFlag_IRES-GFP

    Plasmid

    #198390

    Purpose

    RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EFS-DIO-GRAB_OTmut

    Plasmid

    #185389

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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