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Plasmid#231900PurposedsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.DepositorInsertFrt-Frt14-SD-GFP-SA-Frt-Frt14
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6-CFAPoptim-NAT
Plasmid#221129PurposePlasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMXDepositorInsertFAPoptim
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Esyn-FlpX-TVA950-EYFP-WPRE
Plasmid#234710PurposepAAV transfer plasmid with enhanced Synapsin promoter driving Flp-dependent expression of TVA950-eYFPDepositorInsertTVA950-eYFP
UseAAV; Has fdio cassette for flp-dependent expressi…TagsExpressionMammalianMutationPromoterenhanced SynapsinAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-APOBEC1-EGFP
Plasmid#209324PurposeAAV packaging vector containing a APOBEC1 expression, a P2A-EGFP expression cassette.DepositorInsertAPOBEC1-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAGK58
Plasmid#228118PurposeExpress node 814 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 814, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
N-Terminal Halo XRCC4
Plasmid#207541PurposeHomologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold
Plasmid#226921PurposeEncoding SauriABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nSauriCas9
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Nme2-U6-sgRNA scaffold
Plasmid#226933PurposeEncoding Nme2ABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nNme2Cas9
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold
Plasmid#226935PurposeEncoding SaKKHABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nSaKKHCas9
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKSEe401R
Plasmid#218532PurposeCRISPR/Cas9 genome editing vector pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRRv2_sgTom
Plasmid#218346PurposeCRISPR KO plasmid for tdTomato in human cell linesDepositorInsertInserted sgRNA targetting tdTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK65
Plasmid#228123PurposeExpress node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1550, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK59
Plasmid#228119PurposeExpress node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1429, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK57
Plasmid#228117PurposeExpress node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 482, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK56
Plasmid#228116PurposeExpress node 412 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 412, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK61
Plasmid#228121PurposeExpress node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK60
Plasmid#228120PurposeExpress node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1438, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK62
Plasmid#228122PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK55
Plasmid#228114PurposeExpress node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
UseTagsExpressionMammalianMutationPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Zeo
Plasmid#227271PurposeEmpty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV272
Plasmid#226183PurposeExpresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL475
Plasmid#219503PurposeExpress Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in transDepositorInsertAde2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-APOBEC1-YTH-EGFP
Plasmid#209322PurposeAAV packaging vector containing a APOBEC1-YTH expression, a P2A-EGFP expression cassette,.DepositorInsertAPOBEC1-YTH-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-LB-geneticin
Plasmid#213468PurposeVector to amplify the LB-geneticin fragment for transformation into TSI locus 1DepositorInsertLeft border of the TSI locus 1-split fragment of geneticin cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211844PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter
Plasmid#211847PurposePlant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degronDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoter35SAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPI528
Plasmid#207442Purpose6xHis-SS-TEV-ErkB-T176A-Y178A bacterial expression vector - catalytically inactiveDepositorInsertErkB
UseUnspecifiedTagsExpressionMutationT176A, Y178APromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT58
Plasmid#204304PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT71
Plasmid#204305PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCgVchDonor
Plasmid#203817PurposeDNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertMini-Tn, Spec cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only