Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 16,609 results for: IGH@

Showing: 11701 - 11750 of 16609 results

  3. pSpG-PPE

    Plasmid
    #170130
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9-SpG(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…
    Promoter
    maize Ubiquitin-1
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170130-plasmid-map-sequence-id-333605

    Enlarge

  10. anti-VGluT1 scFv [N28/9] with sortase tag

    Plasmid
    #204424
    Purpose
    Mammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9.
    Depositor
    Jeff Lichtman
    Article
    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d
    Insert
    Recombinant mouse scFv targeting VGluT1 (Rattus norvegicus)
    Use
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204424-plasmid-map-sequence-id-406613

    Enlarge

  14. nCre-ST-NLS-P2A-NLS-pSC-cCre

    Plasmid
    #207638
    Purpose
    A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207638-plasmid-map-sequence-id-409880

    Enlarge

  17. pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

    Plasmid
    #182231
    Purpose
    Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain
    Depositor
    Stéphanie Cabantous
    Article
    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.
    Inserts
    ras homolog family member B (RHOB Human)
    rhotekin RHO binding domain (RBD)
    Use
    Lentiviral
    Tags
    GFP10 (split-GFP tripartite) and GFP11 (M4)
    Expression
    Mutation
    Promoter
    TRE bidirectional
    Available since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    182231-plasmid-map-sequence-id-359620

    Enlarge

  24. pMMK ENTR 1 CMV EYFP Tubulin

    Plasmid
    #206254
    Purpose
    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes EYFP Tubulin under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    EYFP Tubulin
    Use
    Multimate/gateway entr 1
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206254-plasmid-map-sequence-id-406387

    Enlarge

  26. pMMK ENTR 3 CMV mito mCherry

    Plasmid
    #206256
    Purpose
    ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    mito mCherry
    Use
    Multimate/gateway entr 3
    Tags
    COX8 mitochondrial tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206256-plasmid-map-sequence-id-406392

    Enlarge

  30. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139087
    Purpose
    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139087-plasmid-map-sequence-id-273883

    Enlarge

  31. pEB1N-mScarlet-LZ-LOV2

    Plasmid
    #197419
    Purpose
    Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    MAPRE1 (MAPRE1 Human)
    Use
    Tags
    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…
    Expression
    Mammalian
    Mutation
    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197419-plasmid-map-sequence-id-388076

    Enlarge

  33. Ai27

    Plasmid
    #34630
    Purpose
    Cre-dependent expression of hChR2(H134R)-tdTomato, for optogenetic excitation
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.
    Insert
    Floxed hChR2(H134R)-tdTomato
    Use
    Mouse Targeting
    Tags
    tdTomato
    Expression
    Mutation
    His 134 has been mutated to Arg
    Promoter
    CAG
    Available since
    Feb. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    34630-plasmid-map-sequence-id-41505

    Enlarge

  45. pDisplay-GRAB_NPYmut

    Plasmid
    #208678
    Purpose
    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208678-plasmid-map-sequence-id-412165

    Enlarge

  46. pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2

    Plasmid
    #186361
    Purpose
    Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Human Ensconsin (MAP7 Synthetic, Human)
    Use
    Expression of a fluorescent microtubule marker
    Tags
    3xEGFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186361-plasmid-map-sequence-id-373431

    Enlarge

  49. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

    Plasmid
    #198054
    Purpose
    All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning
    Depositor
    Lacra Bintu
    Article
    Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.
    Inserts
    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
    3xFLAG-insert
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G and pEF
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    198054-plasmid-map-sequence-id-391373

    Enlarge

Showing: 11701 - 11750 of 16609 results