We narrowed to 17,761 results for: IGH@
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Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
Tags6His-TEVExpressionBacterialMutationP51APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
TagsHHHHHHENLYFQGExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_Strep
Plasmid#101651PurposeExpression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, Twin-Strep tag
TagsTwin-Strep tagExpressionInsectAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_Strep
Plasmid#101652PurposeExpression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, Twin-Strep tag
TagsTwin-Strep tagExpressionInsectAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMflCT-o4
Plasmid#101313PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o3
Plasmid#101311PurposeContains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.DepositorInsertoriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhT-pHG165a V52A
Plasmid#90050PurposeConstitutive expression of a chimeric LacI:TreR transcription repressorDepositorInsertLLhT/V52A
ExpressionBacterialMutationV52APromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhA-pHG165a Q55A
Plasmid#90052PurposeConstitutive expression of a chimeric LacI:AscG transcription repressorDepositorInsertLLhA/Q55A
ExpressionBacterialMutationQ55APromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhC-pHG165a Q55I
Plasmid#90053PurposeConstitutive expression of a chimeric LacI:CytR transcription repressorDepositorInsertLLhC/Q55I
ExpressionBacterialMutationQ55IPromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
RGD-I27-CC
Plasmid#85393PurposeMTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertRGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
Tags6x HisExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom2.5
Plasmid#87351PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 2.5 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseExpressionBacterial and MammalianPromoter2.5 kbp proximal promoter of human MCT2 (nested d…Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom1.1
Plasmid#87352PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 1.1 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseExpressionBacterial and MammalianPromoter1.1 kbp proximal promoter of human MCT2 (nested d…Available SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom3.2
Plasmid#87349PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 3.2 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseExpressionBacterial and MammalianPromoter3.2 kbp proximal promoter of MCT2 (nested deletio…Available SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-BCL2 3'UTR-mir200c-mut
Plasmid#84598PurposeRenilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding siteDepositorInsertBCL2 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseMutationMutation on miR-200c binding site on BCL2 3'…PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-TOB1 3'UTR-mir200c-mut
Plasmid#84590PurposeRenilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding siteDepositorInsertTOB1 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseMutationMutation on miR-200c binding site on TOB1 3'…PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-RanBP93'UTR-mir200c-mut
Plasmid#84592PurposeRenilla Luciferase reporter assay for RANBP9 3'UTR with mutations on miR-200c binding siteDepositorInsertRANBP9 3'UTR (RANBP9 Human)
UseLuciferaseTagsRenilla LuciferaseMutationMutation on miR-200c binding site on RANBP9 3…PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4-Bre1
Plasmid#73617PurposeControl of the E3 ligase Bre1 with LINXa in yeast.DepositorInsertLINXa4-Bre1dNLS
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL836
Plasmid#62827PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1DepositorInsertsgfp
ipex leader
hfq leader
UseSynthetic BiologyTagsompC leader and st7 RBSExpressionBacterialPromoterpLlacO-1 and pLtetO-1Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1954
Plasmid#62831PurposeLim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1DepositorInsertsM8::ryhB leader
hfq leader
gfp
UseSynthetic BiologyTagsm9::sodB leader and st7 RBSExpressionBacterialPromoterpConNoHind, pLlacO-1, and pLtetO-1Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1969
Plasmid#62832PurposeLim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1DepositorInsertsM8::ryhB leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsm9::sodB leaderExpressionBacterialPromoterpConNoHind, pLlacO-1, and pLtetO-1Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2107
Plasmid#62841PurposeLim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1DepositorInsertsm8::micC leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsm9::ompC leaderExpressionBacterialPromoterpConNoHind, pLlacO-1, and pLtetO-1Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Cx26-7
Plasmid#56156PurposeLocalization: Gap Junctions, Excitation: 604, Emission: 659DepositorInsertCx26
TagsmCardinalExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCardinal-CAF1-C-10
Plasmid#56153PurposeLocalization: Chromatin Assembly Factor 1, Excitation: 604, Emission: 659DepositorInsertCAF1
TagsmCardinalExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdminiSOG-Tubulin-6
Plasmid#57808PurposeLocalization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528DepositorAvailable SinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
PA-mCherry-miniSOG-Vinculin-23
Plasmid#57801PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorAvailable SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
miniSOG-Vinculin-23
Plasmid#57780PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorAvailable SinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCerulean3-Keratin-17
Plasmid#55424PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475DepositorAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Lasp1-N-10
Plasmid#55426PurposeLocalization: Focal Adhesions, Excitation: 433, Emission: 475DepositorAvailable SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-CDC42-C-10
Plasmid#55406PurposeLocalization: Membrane, Excitation: 433, Emission: 475DepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMM2-hPMCA4bct120
Plasmid#47760Purposemammalian expression of human PMCA4 with C-terminal 120aa deletionDepositorInsertPMCA4bct120 (ATP2B4 Human)
ExpressionMammalianMutationdeletion of C-terminal 120 aa (removes calmodulin…PromoterSV40Available SinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-32-Decoy.hPGK.GFP.WPRE
Plasmid#46608DepositorAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-759-Decoy.hPGK.GFP.WPRE
Plasmid#46634DepositorAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-761-Decoy.hPGK.GFP.WPRE
Plasmid#46635DepositorAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-192-Decoy.hPGK.GFP.WPRE
Plasmid#46597DepositorAvailable SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-moesina TAL1
Plasmid#41831DepositorInsertmoesin a (msna Zebrafish)
UseCloning vectorAvailable SinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-moesina TAL2
Plasmid#41832DepositorInsertmoesin a (msna Zebrafish)
UseCloning vectorAvailable SinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIRES:hTRAAK(W262S)
Plasmid#133078PurposeMammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutantDepositorInsertPotassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)
ExpressionMammalianAvailabilityAcademic Institutions and Nonprofits only -
pIRES:hTRAAK(G124I)
Plasmid#133079PurposeMammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutantDepositorInsertPotassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)
ExpressionMammalianMutationG124IAvailabilityAcademic Institutions and Nonprofits only -
pPICZ:hTRAAK(W262S)
Plasmid#130670PurposeP. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFPDepositorInsertKCNK4 (KCNK4 Human)
TagsSNS- 3C_cleavage_site-TAAA-GFPExpressionYeastMutationN104Q, N108Q, W262SAvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7f-WPRE (AAV1)
Viral Prep#104488-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-somBiPOLES-mCerulean (AAV5)
Viral Prep#154951-AAV5PurposeReady-to-use AAV5 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCerulean (Cre-dependent)Available SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7f-WPRE (AAV9)
Viral Prep#104488-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV Retrograde)
Viral Prep#105677-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsFusionRed (Cre-dependent)Available SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-somBiPOLES-mCerulean (AAV9)
Viral Prep#154951-AAV9PurposeReady-to-use AAV9 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCerulean (Cre-dependent)Available SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CKIIa-stGtACR2-FusionRed (AAV Retrograde)
Viral Prep#105669-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsFusionRedAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV pmSyn1-EBFP-Cre (AAV5)
Viral Prep#51507-AAV5PurposeReady-to-use AAV5 particles produced from AAV pmSyn1-EBFP-Cre (#51507). In addition to the viral particles, you will also receive purified AAV pmSyn1-EBFP-Cre plasmid DNA. EBFP-Cre expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterpmSyn1TagsEBFPAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-flex-Voltron-ST (AAV1)
Viral Prep#119036-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hsyn-flex-Voltron-ST (#119036). In addition to the viral particles, you will also receive purified pAAV-hsyn-flex-Voltron-ST plasmid DNA. Synapsin-driven, cre-dependent expression of genetically encoded fluorescent voltage indicator Voltron1 with a soma-targeting sequence. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-DreO-bGHpA (AAV Retrograde)
Viral Prep#50363-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV phSyn1(S)-DreO-bGHpA (#50363). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-DreO-bGHpA plasmid DNA. hSyn-driven Dre recombinase expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean (AAV Retrograde)
Viral Prep#154948-AAVrgPurposeReady-to-use AAV Retrograde particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIIa(0.4)TagsmCeruleanAvailable SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA (AAV1)
Viral Prep#65453-AAV1PurposeReady-to-use AAV1 particles produced from AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA (#65453). In addition to the viral particles, you will also receive purified AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA plasmid DNA. TRE-driven, Cre and Flp-dependent expression of EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromotertetOTagsEGFP (Cre and Flp-dependent)Available SinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only