We narrowed to 11,771 results for: 110

1. pMKO.1-shBirc5

   Plasmid

   #160949

   Purpose

   Birc5 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Birc5 shRNA (Birc5 Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV HA hRB delta CDK + T826

   Plasmid

   #58921

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T826 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with T826 restored

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pGEX-6P-1-INTS3-N

   Plasmid

   #128416

   Purpose

   Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

   Insert

   INTS3 (INTS3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N-terminal region (1–513 aa)

   Promoter

   tac promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFPN1-N-gamma-tubulin (1-333)

   Plasmid

   #87858

   Purpose

   Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Fragment from aa 1 to 333

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTSlb-Q758C

   Plasmid

   #60729

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR_RAF1_94

   Plasmid

   #111826

   Purpose

   Knockout Raf1

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting RAF1 94-114 (RAF1 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSV CDK9 (D167N) HA (P#601)

   Plasmid

   #14639

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

   Insert

   cdk9 D167N (CDK9 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D167N. Kinase negative mutant.

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV HA hRB delta CDK + S249

   Plasmid

   #58908

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S249 restored

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCNL-C1_fibrillarin

   Plasmid

   #65709

   Purpose

   Expresses CNL-fibrillarin in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   fibrillarin (FBL Human)
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

10. 496 pSG5L HA RB del651 (NAAIRS)

    Plasmid

    #10726

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del651 (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 651 to 656 were replaced by NAAIRS, a fl…

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

11. 500 pSG5L HA RB del685 (NAAIRS)

    Plasmid

    #10729

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del685 (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 685 to 690 were replaced by NAAIRS, a fl…

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-FLAG-NEMO-C54/347A

    Plasmid

    #27268

    Depositor

    Thomas Gilmore

    Article

    Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

    Insert

    NEMO (IKBKG Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cys54Ala Cys347Ala

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

13. TMED1-bio-His

    Plasmid

    #51832

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED1 (TMED1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST27-GST-ATXN1 FL(2Q)

    Plasmid

    #21752

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains 2Q (2 glutamines) and there isno …

    Promoter

    CMV

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pSN69

    Plasmid

    #100125

    Purpose

    expresses putative cMYC DBD with a HA tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Insert corresponds to the DBD of cMYC

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. YFP-Fibrillarin-C1

    Plasmid

    #134538

    Purpose

    Expresses YFP-Fibrillarin fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET22b-ecDHFR-N23PP/L28F/G51PEKN

    Plasmid

    #118585

    Purpose

    Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, L28 mutated to F28, G51 mutat…

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLINK CDK9 myc (D167N) (P#1084)

    Plasmid

    #14637

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

    Insert

    cdk9 D167N (CDK9 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D167N. Kinase negative mutant.

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pTSara-R756K

    Plasmid

    #60727

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tdEos-Farnesyl-5

    Plasmid

    #57613

    Purpose

    Localization: Plasma Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits