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pCellFree_G10

Plasmid

#67144

Purpose

Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and sfGFP

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Luc-Pten-3'UTR

Plasmid

#64098

Purpose

Pten Luciferase reporter

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

Pten 3'UTR (PTEN Human)

Expression

Mammalian

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only
pBT138b-proA

Plasmid

#138514

Purpose

P3g

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSB3K3-IC15

Plasmid

#165408

Purpose

Bacteria gene circuit on medium copy backbone.

Depositor

Xiaojun Tian

Article

Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

Insert

IC15 gene circuit

Expression

Bacterial

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-spGFP(11)

Plasmid

#190257

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Jack Sunter

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

spGFP(11)

Use

For endogenous gene tagging

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJZC523

Plasmid

#62320

Purpose

sgRNA for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Expression

Yeast

Promoter

SNR52

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP

Plasmid

#84256

Purpose

Expresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarlet

Plasmid

#179818

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mNG

Plasmid

#179821

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pKM394

Plasmid

#134211

Purpose

Baculovirus expression of CENP-N and CENP-L

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N-his-CENP-L

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MLS- mir-17-19b

Plasmid

#64089

Purpose

expresses a truncated version of mir-17-92 lacking miR-92

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

mir-17-19b (MIR17 Human)

Expression

Mammalian

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 9

Plasmid

#8476

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

1191RVYDRR1196-DAAADD (R1191D, V1192A, Y1193A, D1…

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only
rNL1(46-638)

Plasmid

#20172

Depositor

Axel Brunger

Article

Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

Insert

Neuroligin 1 (Nlgn1 Rat)

Tags

C-terminal 6X His tag

Expression

Insect

Mutation

no splice sites

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsY14_I

Plasmid

#146583

Purpose

Mammalian Expression of HsY14

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HsY14 (RBM8A Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarletI

Plasmid

#201442

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP

Plasmid

#84255

Purpose

Expresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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YSBE 596 : pNAT_mCherry

Plasmid

#92088

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, NAt selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

mCherry

Use

tagging proteins in cryptococcus neoforman

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF17S

Plasmid

#35414

Depositor

Jeremy Wang

Article

Pan et al Development. 2005 Sep;132(18):4029-39. E

Insert

Rnf17s (Rnf17 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only
pSW596 - mTurq2

Plasmid

#115985

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

mTurquoise2

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 4

Plasmid

#8474

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

391SQ392-PA (S391P, Q392A)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only