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pCW57.1-pDUXChd:hDUX4ctd-ca

Plasmid

#198237

Purpose

Lentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

DUX4L pDUXChd:hDUX4ctd (codon altered) (DUX4 S. scrofa (porcine), Human)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1-pDUXChd:hDUX4ctd-ca

Plasmid

#198237

Purpose

Lentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

DUX4L pDUXChd:hDUX4ctd (codon altered) (DUX4 S. scrofa (porcine), Human)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) HA-TAT-tag pET

Plasmid

#8526

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

HA

Expression

Bacterial

Mutation

replace T7 and HIS tags of pET with an HA tag;

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB4 (human) HA-TAT-tag pET

Plasmid

#8526

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

HA

Expression

Bacterial

Mutation

replace T7 and HIS tags of pET with an HA tag;

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
BII-gR-PtW-eSpCas9

Plasmid

#133357

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

HA-tagged eSpCas9

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-gR-PtW-eSpCas9

Plasmid

#133357

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

HA-tagged eSpCas9

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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SIN-CMV-Cas9-V5-WPRE

Plasmid

#87904

Purpose

To produce lentiviral vector for gene editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

Cas9-V5

Use

Lentiviral

Tags

V5

Mutation

human codon-optimized

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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SIN-CMV-Cas9-V5-WPRE

Plasmid

#87904

Purpose

To produce lentiviral vector for gene editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

Cas9-V5

Use

Lentiviral

Tags

V5

Mutation

human codon-optimized

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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TRRAP sgRNA1

Plasmid

#138182

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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TRRAP sgRNA1

Plasmid

#138182

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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TRRAP sgRNA2

Plasmid

#138183

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TRRAP sgRNA2

Plasmid

#138183

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF17 tail 400-1038

Plasmid

#134617

Purpose

Mammalian expression of mouse KIF1A 400-1038

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF17 (Kif17 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 399 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF17 tail 400-1038

Plasmid

#134617

Purpose

Mammalian expression of mouse KIF1A 400-1038

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF17 (Kif17 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 399 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-ER-E2F2-dRb

Plasmid

#62805

Purpose

expresses an inducible E2F2 mutant that lacks the Rb binding domain

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

E2F2 (E2F2 Human)

Use

Retroviral

Tags

HA-Er

Expression

Mammalian

Mutation

deletion of amino acids 302-437, including the Rb…

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-ER-E2F2-dRb

Plasmid

#62805

Purpose

expresses an inducible E2F2 mutant that lacks the Rb binding domain

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

E2F2 (E2F2 Human)

Use

Retroviral

Tags

HA-Er

Expression

Mammalian

Mutation

deletion of amino acids 302-437, including the Rb…

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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SIN-PGK-Cas9-WPRE

Plasmid

#87886

Purpose

To produce Lentiviral vector for gene editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

hCas9

Use

Lentiviral

Tags

nls

Mutation

Human codon-optimized

Promoter

mouse PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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SIN-PGK-Cas9-WPRE

Plasmid

#87886

Purpose

To produce Lentiviral vector for gene editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

hCas9

Use

Lentiviral

Tags

nls

Mutation

Human codon-optimized

Promoter

mouse PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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