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pRS426-TUB1-internal6xHis (1-440)

Plasmid

#60391

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4), amino a…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
M34 Xdsh PDZ bait-pGBT9

Plasmid

#17190

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Xdsh (dvl2.L Frog)

Tags

Gal4-DBD

Expression

Yeast

Mutation

Dsh bp 343-1191

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pThom77.2

Plasmid

#101646

Purpose

Encodes the fluorescent protein amFP486 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Dectin1A-N-10

Plasmid

#55414

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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K349CLM-6His 215/347

Plasmid

#24451

Depositor

Ron Vale

Article

Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

Insert

Kinesin (KIF5B Human)

Tags

His

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S, E215C, E347C

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
XE160 XDshHA-pt mutant PDZ-CS2+

Plasmid

#16780

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Dishevelled (dvl2.L Frog)

Use

Xenopus expression

Tags

HA

Mutation

point mutation in the binding pocket of the PDZ b…

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
XE161 XDshHA-triple pt mutant-CS2+

Plasmid

#16779

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Dishevelled (dvl2.L Frog)

Use

Xenopus expression

Tags

HA

Mutation

triple point mutation outside of the PDZ binding …

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
mCerulean3-Dectin1A-C-10

Plasmid

#55413

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB2a Cterminal tail

Plasmid

#60396

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pAAV.hSynap.(cyto).iATPSnFR2.A95A.A119L.mCherry

Plasmid

#244920

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mCherry

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mCherry

Use

AAV

Mutation

A95A.A119L

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iATPSnFR2.A95K.tdTomato

Plasmid

#244921

Purpose

iATPSnFR2.A95K variant with non-responsive tdTomato

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.tdTomato

Use

AAV

Mutation

A95K

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAMBIA1302-Pro35S:Irp9

Plasmid

#246319

Purpose

Overexpression of a bacterial bifunctional salicylate synthase targeted to the cytosol

Depositor

Chung-Jui Tsai

Article

Xue et al Plant Cell. 2013 Jul;25(7):2714-30. doi:

Insert

Irp9

Expression

Plant

Promoter

cauliflower mosaic virus 35S

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCAMBIA1302-Pro35S:Fd-Irp9

Plasmid

#246318

Purpose

Overexpression of a bacterial bifunctional salicylate synthase, Irp9, targeted to the chloroplast

Depositor

Chung-Jui Tsai

Article

Xue et al Plant Cell. 2013 Jul;25(7):2714-30. doi:

Insert

Fd-Irp9

Expression

Plant

Promoter

cauliflower mosaic virus 35S

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-HyPerFLEX-NLSx3-WPRE

Plasmid

#217563

Purpose

Mammalian expression of HyPerFLEX targeted to the nucleus

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NLS

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CaMKII-HyPerFLEX-UnaG-WPRE

Plasmid

#217564

Purpose

Expression of HyPerFLEX fused with UnaG in mammalian neurons

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-HyPerFLEX-UnaG-WPRE

Plasmid

#217565

Purpose

Mammalian expression of HyPerFLEX fused with UnaG

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-UnaG

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-HyPerFLEX-NES-WPRE

Plasmid

#217562

Purpose

Mammalian expression of HyPerFLEX targeted to the cytoplasm

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-NES

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-HyPerFLEX-Sec61b

Plasmid

#238940

Purpose

Mammalian expression of HyPerFLEX targeted to the cytosolic side of the endoplasmic reticulum membranes

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-Sec61b

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pC1-HyPerFLEX-ERlum

Plasmid

#238941

Purpose

Mammalian expression of HyPerFLEX targeted to the luminal side of the endoplasmic reticulum membranes

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-ERlum (Calreticulin signal sequence-HyPerFLEX-KDEL)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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2xmyc-RILPL1

Plasmid

#244982

Purpose

Express RILPL1 in mammalian cells with 2xmyc tag

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

RILPL1 (RILPL1 Human)

Tags

2xmyc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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