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pBSV2G_PflaB-msfYFPBb

Plasmid

#118233

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfYFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mCeruleanBb

Plasmid

#118235

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCerulean

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCerulean

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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DT035A

Plasmid

#159397

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tac promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SpSuc1

Plasmid

#162558

Purpose

Expresses GST-tagged S.pombe Suc1 in E.coli

Depositor

Iain Hagan

Article

Singh et al Methods Mol Biol. 2021;2329:123-142. doi

Insert

Schizosaccharomyces pombe Suc1 (suc1 Fission Yeast)

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PhoCl-MBP

Plasmid

#87689

Purpose

Express PhoCl-MBP in E.coli

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl fused to Maltose-Binding Protein

Expression

Bacterial

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-BirA

Plasmid

#71753

Purpose

U+36GFP-BirA for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)_BirA_6XHis_LPETG

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pIntegrase_10

Plasmid

#60581

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_10

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mEGFPBb

Plasmid

#118234

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mEGFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Aurein1.2_(pos36GFP)-Cys

Plasmid

#71757

Purpose

Aurein1.2_+36GFP-Cys expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2_(pos36_GFP)-Cys

Tags

6XHis

Expression

Bacterial

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pNH082

Plasmid

#42165

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Expression

Worm

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pEBB HA BIR 3

Plasmid

#25689

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

aa 262-350 (BIR 3)

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pICSL70002

Plasmid

#50333

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pJL1 Trx-Bx

Plasmid

#106290

Purpose

Expresses Trx-Bx in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Trx-BX

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB HA XIAP H467A

Plasmid

#25693

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

H467A

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pBSV2G_PflaB-mCitrineBb

Plasmid

#118236

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCitrine

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCitrine

Expression

Bacterial

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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(GGS)9_(+36GFP)-Cre.ape

Plasmid

#71747

Purpose

+36-GFP-Cre fusion expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_+36GFP-Cre

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pEBB HA BIR 2-3

Plasmid

#25691

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

amino acid 124RDH-LEE350 (BIR 2&3)

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
7xNLS-pMJ915v2-sfGFP

Plasmid

#88922

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfCFPBb

Plasmid

#118232

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfCFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfCFP

Expression

Bacterial

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET20b- hTPI Lys13Arg

Plasmid

#50726

Purpose

overexpression of the human TPI1 Lys13Arg allele in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Tags

6xHIS tag

Expression

Bacterial

Mutation

changed Lysine 13 to Argenine

Promoter

T7

Available Since

Feb. 1, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2_P0526-mCherryBb

Plasmid

#118241

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0526 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0526 promoter driven mCherry

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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mRFP1-36

Plasmid

#63825

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb4EIG

Plasmid

#184683

Purpose

NIR-Fb to E.coli dihydrofolate reductase

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb4EIG

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEBB FLAG XIAP D148A/W310A

Plasmid

#25685

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

FLAG

Expression

Mammalian

Mutation

D148A W310A

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pEBB HA XIAP delta BIR

Plasmid

#25686

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

delta BIR Contains only aa 331-497

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
DT036A

Plasmid

#159396

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tet promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tet-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tet promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEBB XIAP Delta RING

Plasmid

#40828

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Expression

Mammalian

Mutation

Delta RING: contains aa 1-448

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pEBB XIAP H467A

Plasmid

#25680

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Expression

Mammalian

Mutation

H467A

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pNH013

Plasmid

#42147

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Use

Synthetic Biology

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB XIAP D148A/W310A

Plasmid

#25678

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Expression

Mammalian

Mutation

D148A W310A

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
mRFP1-1

Plasmid

#63816

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mRFP1-9

Plasmid

#63818

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Aurein1.2_(+36GFP)-BirA

Plasmid

#71752

Purpose

Aurein1.2+36GFP-BirA fusion for E.Coli expression

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2+36GFP-BirA

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH078

Plasmid

#42164

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Expression

Worm

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB FLAG XIAP D148A

Plasmid

#25683

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

FLAG

Expression

Mammalian

Mutation

D148A

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pET20b- hTPI Ile170Val

Plasmid

#50724

Purpose

overexpression of the human TPI1 Ile170Val allele in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Tags

6xHIS tag

Expression

Bacterial

Mutation

changed Isoleucine 170 to Valine

Promoter

T7

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB-HA-hILP del(2XBIR)

Plasmid

#25676

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

aa 262-497

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
1xNLS-pMJ915v2-sfGFP

Plasmid

#88919

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 9401 - 9440 of 10368 results

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