We narrowed to 9,888 results for: Coli

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pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500

Plasmid

#92228

Purpose

Expression of zmADH using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

NTerm 6xHis Tagged zmADH

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1 S222TAG

Plasmid

#68302

Purpose

N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 S222TAG (MAP2K1 Human, Synthetic)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-FGF21

Plasmid

#182407

Purpose

Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-fgf21 (FGF21 Human)

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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R26TV LSL-TRL

Plasmid

#68476

Purpose

Rosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassette

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

tTR-KRAB-2A-rtTA3-2A-Luciferase

Use

Cre/Lox, Luciferase, and Mouse Targeting

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV iGluf

Plasmid

#105833

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25D variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Glu 25 to Asp

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-TetR

Plasmid

#103797

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-TetR (CIB1, tetR E. coli, Mustard Weed)

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1 S218TAG

Plasmid

#68301

Purpose

N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 S218TAG (MAP2K1 Human, Synthetic)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-StefinA

Plasmid

#182403

Purpose

Bacterial expression of Vesicle Nucleating peptide-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-stefin (CSTA Human)

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBXNPHM3-Nb_MsbA#1

Plasmid

#186428

Purpose

Plasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1

Depositor

Markus Seeger

Article

Galazzo et al Sci Adv. 2022 Oct 14;8(41):eabn6845. doi

Insert

Nanobody Nb_MsbA#1

Use

Affinity Reagent/ Antibody

Tags

3C cleavage site, His Tag, Maltose Binding Protei…

Promoter

pBAD

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSBK.013

Plasmid

#187213

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500

Plasmid

#92317

Purpose

Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

Optimized NTerm 6xHis tagged monomeric RFP (mRFP)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pPPC001

Plasmid

#171138

Purpose

For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Sp.pCas9, BBa_J23107

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSBK.014

Plasmid

#187215

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDN-G1Tt

Plasmid

#44509

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
tetR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pZJQIBEBT003

Plasmid

#140053

Purpose

The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

Depositor

Jia Zhang

Article

Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

Insert

eMutS-L157C-G233C-CBM-EGFP

Tags

His-Tag

Expression

Bacterial

Mutation

L157C, G233C

Promoter

T7

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSBK.009

Plasmid

#187209

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG])
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBC2T-Ypet-FtnA

Plasmid

#108969

Purpose

expresses BC2-tag-Ypet-FtnA in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

FtnA (ftnA Escherichia coli)

Tags

BC2-tag and Ypet

Expression

Mammalian

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-G-S18

Plasmid

#127825

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-agrBD-IV

Plasmid

#53440

Purpose

Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus (with D29Y mutation in agrD)

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

D to Y mutation of residue 29 of the agrD gene (c…

Promoter

T7-lac

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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