We narrowed to 22,240 results for: NOL

1. NM1-5S-tRNA-SgH

   Plasmid

   #128178

   Purpose

   Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   gRNA cloning cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   5S rRNA-tRNA(Arg)

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAC446

   Plasmid

   #195239

   Purpose

   Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b-His-RF2

   Plasmid

   #124130

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 2 (prfB Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD.Sigma32.I54N

   Plasmid

   #59982

   Purpose

   Expresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environment

   Depositor

   Jeffery Kelly

   Article

   Zhang et al ACS Chem Biol. 2014 Jul 22.

   Insert

   Sigma32 I54N
   

   Expression

   Bacterial

   Mutation

   I54N

   Available Since

   Nov. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTK229

   Plasmid

   #71256

   Purpose

   luciferase reposter with 229 bp of the HSV TK promoter

   Depositor

   Peter Cockerill

   Article

   Hawwari et al J Immunol. 2002 Aug 15;169(4):1876-86.

   Insert

   Thymidine Kinase promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   HSV Thymidine Kinase

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE60-RRF-His

   Plasmid

   #124132

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Ribosome recycling factor (frr Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. EasyFusion T2A-H2B-miRFP703

   Plasmid

   #113097

   Purpose

   General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-miRFP703
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBT280

   Plasmid

   #122610

   Purpose

   express evoFERNY-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoFERNY-BE4max
   

   Expression

   Mammalian

   Mutation

   H102P D104N

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG1188 mCherry-cagedFIREtag

   Plasmid

   #205177

   Purpose

   Expresses mCherry-cagedFIREtag in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   mCherry-FIREtag-DEVDG-MoA
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONRPEX18Tp-SceI-pheS

    Plasmid

    #111608

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGNW2

    Plasmid

    #122086

    Purpose

    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRMPVIR

    Plasmid

    #27994

    Purpose

    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.

    Depositor

    Scott Lowe

    Article

    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

    Insert

    TRMPVIR (shRen)
    

    Use

    RNAi and Retroviral; Fluorescent reporters

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG1171 FIREmate-ECFP-Giantin

    Plasmid

    #205166

    Purpose

    Expresses FIREmate-ECFP-Giantin in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    FIREmate-ECFP-Giantin
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. RSVpGL3 (RSV promoter-luciferase in pGL3-basic)

    Plasmid

    #40343

    Depositor

    Alexander Dent

    Article

    Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

    Insert

    RSV promoter
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    RSV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET30-T7-sfGFP

    Plasmid

    #180757

    Purpose

    pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Tags

    strep tag

    Expression

    Bacterial

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG1237 FIREmate-ECFP-Fis1

    Plasmid

    #205173

    Purpose

    Expresses FIREmate-ECFP-Fis1 in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    FIREmate-ECFP-Fis1
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTNS2

    Plasmid

    #87802

    Purpose

    For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. JD553

    Plasmid

    #160392

    Purpose

    Wheat GRF4-GIF1 chimera in pDONR-zeo

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    wheat GRF4-GIF1 chimera
    

    Use

    Entry clon

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_SpG‐ABE8e(V106W)

    Plasmid

    #187069

    Purpose

    ABE8e(V106W)-SpG

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    SpG‐ABE8e(V106W)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 4xNLS-pMJ915v2

    Plasmid

    #88917

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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