We narrowed to 25,023 results for: Nes

1. pRD405

   Plasmid

   #163110

   Purpose

   Expression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNA

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS_mScarlet-I_H-NSdbd_NLS
   

   Use

   Eukaryotic expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter; T7 promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. G-GECO1.2-Orai1

   Plasmid

   #73562

   Purpose

   Green fluorescent reporter of Orai1-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   G-GECO1.2-Orai1 (ORAI1 Human, Synthetic)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immediate Early

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUb-Cas9-EGFP_cU6:6-sgRNA

   Plasmid

   #190597

   Purpose

   The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   EGFP - separated by T2A

   Expression

   Mutation

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Orai1-GCaMP6f

   Plasmid

   #73564

   Purpose

   Green fluorescent reporter of Orai1-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   Orai1-GCaMP6f (ORAI1 Human, Synthetic)
   

   Use

   Tags

   GCaMP6f

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immediate Early

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-FLEX-SaCas9-U6-sgGrin1

   Plasmid

   #124852

   Purpose

   Mutagenesis of Grin1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin1 (Grin1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-CR033

   Plasmid

   #234042

   Purpose

   expresses ABE8e(V106W) base editor in a human T cell optimized lentiviral transfer plasmid

   Depositor

   Alexander Marson

   Article

   Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

   Insert

   TadA8e(V106W)-Cas9(D10A)-P2A-blast
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEN_TT 3xFLAG ID2

   Plasmid

   #98390

   Purpose

   Gateway entry vector for inducible expression of human ID2 with N-terminal 3xFLAG tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   ID2 (ID2 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   TRE tight promoter

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV5 WT KSR1

   Plasmid

   #25970

   Depositor

   Rob Lewis

   Article

   Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

   Insert

   Kinase suppressor of Ras 1 (Ksr1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. tet_pLKO.1_puro_shNRF2 #2

   Plasmid

   #136585

   Purpose

   Expresses an inducible short hairpin targeting human NRF2 sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shNRF2v2 (NFE2L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid

    #190598

    Purpose

    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    mCherry - separated by T2A

    Expression

    Mutation

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pN1-RnKIF5C(1-560)-Halo-Flag

    Plasmid

    #188071

    Purpose

    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

    Depositor

    Kristen Verhey

    Article

    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

    Insert

    rat KIF5C (Kif5c Rat)
    

    Use

    Tags

    Flag and Halo

    Expression

    Mammalian

    Mutation

    aa1-560

    Promoter

    CMV

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR221-YAP1 CA

    Plasmid

    #79502

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    3XFLAG

    Expression

    Mutation

    S127A

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pB-Gaolf-Integration

    Plasmid

    #129456

    Purpose

    piggyBac transposon vector that Inducibly (Tet-On) expresses Gaolf

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    G alpha olf (Gnal Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. dCas9-ddMSK1

    Plasmid

    #165603

    Purpose

    Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 Human, Synthetic, S. Pyogenes)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114103

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi

    Insert

    sbGLuc-VChR1-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    motoneuron-specific Homeobox 9 promoter

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR221-EF1.Pro

    Plasmid

    #79488

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    EF1 Promoter (EEF1A1 Human, Synthetic)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    EF1 Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBabe JUND-HA neo

    Plasmid

    #58489

    Purpose

    mammalian expression of human JUND

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JUND (JUN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR221-IL6.Pro

    Plasmid

    #79491

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Il6 Promoter (IL6 Human, Synthetic)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    IL6 Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. G-GECO1-Orai1

    Plasmid

    #73561

    Purpose

    Green fluorescent reporter of Orai1-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    NM_032790.3 (ORAI1 Human, Synthetic)
    

    Use

    Tags

    G-GECO1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSP2440

    Plasmid

    #72250

    Purpose

    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge