We narrowed to 6,250 results for: nls

1. pC130: pAAV.CMV-CasRx-VEGFA sgRNA

   Plasmid

   #203443

   Purpose

   Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA
   RfxCas13d
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pM148: pAAV.CMV-Cas13e-NT sgRNA

   Plasmid

   #203446

   Purpose

   Plasmid expressing active RfxCas13d with non-targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-non targeting (NT) sgRNA
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

   Plasmid

   #203447

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM150: pAAV.CMV-Cas13e-VEGFA sgRNA array

   Plasmid

   #203448

   Purpose

   Plasmid expressing active Cas13e with an array of three VEGFA mRNA targeting gRNAs

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA array
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-rTetR(SE)-DNMT3Bcd-2A-mTurquoise

   Plasmid

   #225693

   Purpose

   Lentiviral expression of rTetR(SE) fused to the catalytic domain of DNMT3B and mTurquoise-NLS

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   rTetR-DNMT3B catalytic domain (DNMT3B Human, Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG and T2A-mTurquoise

   Expression

   Mammalian

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-rTetR(SE)-EED-2A-mTurquoise

   Plasmid

   #225692

   Purpose

   Lentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLS

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   rTetR-EED (EED Human, Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG and T2A-mTurquoise

   Expression

   Mammalian

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-rTetR(SE)-HP1a-2A-mTurquoise

   Plasmid

   #225691

   Purpose

   Lentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLS

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   HP1a (CBX5 Human, Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG and T2A-mTurquoise

   Expression

   Mammalian

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177360

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Promoter

   Synapsine promoter and U6 promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177365

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Promoter

   CMV and U6 promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-CMV-scFVDnmt3L_bGHpA

    Plasmid

    #177350

    Purpose

    AAV expression of scFV-fused C terminal domain of Dnmt3l for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    194–415 aa

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CMV-scFVtet2_bGHpA

    Plasmid

    #177352

    Purpose

    AAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    Catalytic domains of human TET2 (1129–1936 and 14…

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-hSyn-scFVtet2_bGHpA

    Plasmid

    #177353

    Purpose

    AAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    Catalytic domains of human TET2 (1129–1936 and 14…

    Promoter

    human Synapsine promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-TRE-scFVtet2_bGHpA

    Plasmid

    #177358

    Purpose

    AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    Catalytic domains of human TET2 (1129–1936 and 14…

    Promoter

    Tetracycline-dependent promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. B2M-SDMutation-EhT

    Plasmid

    #215546

    Purpose

    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    

    Use

    CRISPR

    Mutation

    The second base of the first intron of B2M (From …

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

    Plasmid

    #212738

    Purpose

    Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-NLS-VSV and myc

    Expression

    Yeast

    Mutation

    HOIP(C885A)

    Promoter

    ADH1

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. HypaR-SpCas9

    Plasmid

    #126757

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Hypa2SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    CBh

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAB(EXPR-HDAC5-trun1)

    Plasmid

    #114400

    Purpose

    For PYL-HDAC5 aa 1-275 expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5 aa 1-275 (HDAC5 Human)
    

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGG431

    Plasmid

    #165615

    Purpose

    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Corrected homology relative to wild type SpCas9 (…

    Promoter

    TEF1

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. deltaVP1 + VP2C-GFP-MIOX

    Plasmid

    #166682

    Purpose

    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFP-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. deltaVP1 + VP2C-GFPdeg

    Plasmid

    #166677

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFPdeg
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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