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Plasmid#205004PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvACT-mEGFP-NLS
Plasmid#205001PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Ramazzottius varieornatus
UseTardigrade expressionAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-GFP
Plasmid#198589PurposeGateway Entry CloneDepositorInsertGFP
UseGateway CloningMutationNone (wildtype)Available SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BALF2
Plasmid#198578PurposeGateway Entry CloneDepositorAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hRedOpsin-fCX3CL1
Plasmid#164421PurposeAAV plasmid expressing full-length CX3CL1 (fractalkine) in photoreceptorsDepositorAvailable SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-hRedOpsin-fCD200
Plasmid#164425PurposeAAV plasmid expressing full-length CD200 in photoreceptorsDepositorAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 mitochondrial matrix
Plasmid#172063PurposeUAS vector to express GFP1-10 in the mitochondrial matrixDepositorInsertGFP1-10 mitochondrial matrix
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)
Plasmid#172066PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intronDepositorInsertmTagBFP2(11x7)
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAC-U63-QtgRNA2.1-8R
Plasmid#170514PurposegRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6:3Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGC(2.1)-U6.3
Plasmid#170516PurposePCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFPDepositorInsertgRNAcore(2.1)-pU6.3
UsePcr template vector for amplifying grnacore(2.1)-…Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1-M7A
Plasmid#159167PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1-M7A
TagsSV40 NLSExpressionYeastMutationMutated Met 7 of the cyt b562 module of nuc-yHS1 …PromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-nef-CIAO-TVA-mCherry
Plasmid#149295PurposeCre-dependent expression of TVA:mCherry fusion protein for rabies tracing.DepositorInsertTVA:mCherry
UseAAVMutationStart codon removed.Available SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
SOD1-VC
Plasmid#89758Purposeexpresses human SOD1 fused to the C-terminal part of VenusDepositorAvailable SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
iTol2Amp-γ-crystallin:venusGFP
Plasmid#108462PurposeThis plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.DepositorInsertiTol2Amp crystGFP
Available SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluf
Plasmid#106121PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR E25D variant
TagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromotersynapsinAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS_V65I
Plasmid#98567PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
Mutated ATP-dependent Clp protease adapter protein
ExpressionBacterialMutationContains the V65I mutation that increases discrim…Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL-CP
Plasmid#65715PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
UseLuciferaseAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-YNL-CP
Plasmid#65714PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
UseLuciferaseAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
CFP-GIV-CT WT
Plasmid#69532PurposeMammalian expression of The CFP-tagged GIV-CT biosensorsDepositorInsertGIV (CCDC88A Human)
TagsCFPExpressionMammalianMutationC terminal amino acids S1660-S1870 of human GIVPromoterCMVAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD438
Plasmid#63759Purposeeae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.DepositorInserteae
ExpressionBacterialAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only