We narrowed to 8,355 results for: construct

1. pET-MBP-creM

   Plasmid

   #112238

   Purpose

   pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM

   Depositor

   Emily Balskus

   Article

   Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

   Insert

   CreM
   

   Use

   Tags

   MBP

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMbd3-GGSG-AID-3xF_PP

   Plasmid

   #235507

   Purpose

   Targeting construct to make C-term fusion with GGSG-AID-3xFLAG, roxed Pgk-Puro

   Depositor

   Brian Hendrich

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Mouse Targeting

   Tags

   AID, 3xFLAG

   Expression

   Mutation

   None

   Promoter

   PGK

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pChd8_GGSG_Fkbp_2xHA_BB

   Plasmid

   #235524

   Purpose

   Targeting construct: Chd8-FKBP-HA2 with Roxed Blasto cassette

   Depositor

   Brian Hendrich

   Insert

   Chd8 (Chd8 Mouse)
   

   Use

   Mouse Targeting

   Tags

   FKBP, 2xHA

   Expression

   Mutation

   None

   Promoter

   beta actin

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10

   Plasmid

   #219701

   Purpose

   Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1

   Depositor

   Jennifer Nemhauser

   Insert

   pAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2/shαSMA/GFP4_Seq1/clone1

   Plasmid

   #206138

   Purpose

   Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Insert

   alpha-SMA (Acta2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCASCADE-gltA1-gltA2-udhA-zwf

   Plasmid

   #87152

   Purpose

   gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   gltA1-gltA2-udhA-zwf guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCASCADE-fabI-gltA1-gltA2-gapAP1

   Plasmid

   #87147

   Purpose

   fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   fabI-gltA1-gltA2-gapAP1 guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-multi-Puro (pRW1441)

   Plasmid

   #216217

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. STING-V1

   Plasmid

   #124262

   Purpose

   C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

   Depositor

   Eric Schirmer

   Insert

   TMEM173 (STING1 Human)
   

   Use

   Tags

   Venus1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mu-FLT3Lv1-pTRIP

    Plasmid

    #163519

    Purpose

    Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine-FLT3L variant 1 (Flt3l Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. V1-STING

    Plasmid

    #124263

    Purpose

    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

    Depositor

    Eric Schirmer

    Insert

    TMEM173 (STING1 Human)
    

    Use

    Tags

    Venus1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. STING-V2

    Plasmid

    #124264

    Purpose

    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

    Depositor

    Eric Schirmer

    Insert

    TMEM173 (STING1 Human)
    

    Use

    Tags

    Venus2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. V2-STING

    Plasmid

    #124265

    Purpose

    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

    Depositor

    Eric Schirmer

    Insert

    TMEM173 (STING1 Human)
    

    Use

    Tags

    Venus2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-mNG-IRES-mScarlet-dMCP

    Plasmid

    #231089

    Purpose

    Bicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.

    Depositor

    John Ngo

    Insert

    mNG-IRES-mScarlet-dMCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2-CAGIG-WGA-mCherry

    Plasmid

    #224119

    Purpose

    To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.

    Depositor

    Pantelis Tsoulfas

    Insert

    WGA-mCherry
    

    Use

    AAV

    Tags

    WGA and mCherry

    Expression

    Mammalian

    Mutation

    All mutations related to the WGA in this construc…

    Promoter

    CAGIG

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CROPseq-multi-Zeo (pRW1445)

    Plasmid

    #216218

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Zeocin resistance

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLIK CXADR-V5 hygro

    Plasmid

    #220362

    Purpose

    Lentiviral expression vector for an inducible V5-tagged CXADR construct

    Depositor

    Kevin Janes

    Insert

    CXADR (CXADR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MDH1-PGK-GFP_2.0

    Plasmid

    #11375

    Purpose

    Retroviral construct for miRNA expression

    Depositor

    Chang-Zheng Chen

    Article

    Chen et al Science. 2004 Jan 2. 303(5654):83-6.

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pG20_Venus_Hyg

    Plasmid

    #159703

    Purpose

    pGREEN-based cloning construct for expression in planta

    Depositor

    Hans-Henning Kunz

    Article

    Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

    Type

    Empty backbone

    Use

    Tags

    mVenus

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-mNG-IRES-mScarlet-dPCP

    Plasmid

    #231090

    Purpose

    Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.

    Depositor

    John Ngo

    Insert

    mNG-IRES-mScarlet-dPCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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