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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 4741 - 4760 of 14582 results

  1. pU6_HEK3_nsgRNA(PP7)

    Plasmid
    #232444
    Purpose
    nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Publication
    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter
    Use
    CRISPR
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232444-plasmid-map-sequence-id-462757

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  2. pC3.1_CMV_oROS-G

    Plasmid
    #216111
    Purpose
    Expresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.
    Depositor
    Andre Berndt
    Publication
    Lee et al bioRxiv [Preprint]. 2024 Feb 4:2024.01.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    oROS-G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216111-plasmid-map-sequence-id-434849

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  4. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  5. pMP597

    Plasmid
    #134999
    Purpose
    Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    Depositor
    Ahmad Khalil
    Publication
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134999-plasmid-map-sequence-id-267926

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  9. pMOD_C2616

    Plasmid
    #91090
    Purpose
    Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    At7SL
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91090-plasmid-map-sequence-id-182055

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  13. pCAG-AsCas12a-Plus-mCherry

    Plasmid
    #191231
    Purpose
    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity
    Depositor
    Zhili Rong
    Publication
    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AsCas12a-Plus-P2A-mCherry
    Use
    CRISPR
    Mutation
    E174R, R951K, R955A
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191231-plasmid-map-sequence-id-392988

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  14. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162680-plasmid-map-sequence-id-321455

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  18. LP580

    Plasmid
    #90205
    Purpose
    pCMV-myc with cDNA encoding HsTRAPPC13 full length
    Depositor
    Lotte Pedersen
    Publication
    Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRAPPC13 (TRAPPC13 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    full length protein, no mutations
    Promoter
    CMV
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90205-plasmid-map-sequence-id-178315

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Showing: 4741 - 4760 of 14582 results