We narrowed to 6,250 results for: nls

1. pLV-dt/deltaEGFP

   Plasmid

   #163919

   Purpose

   PGK-driven dTomato expression. Lacks the CBA-promoter controlling EGFP expression.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   dtomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. prCOrev

   Plasmid

   #153961

   Purpose

   Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
   

   Use

   Retroviral; A reporter plasmid detecting dna reco…

   Tags

   NLS-ZF

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   LTR

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. prCO

   Plasmid

   #153960

   Purpose

   Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
   

   Use

   Retroviral; A reporter plasmid detecting dna reco…

   Tags

   NLS-ZF

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   LTR

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJW1841

   Plasmid

   #154338

   Purpose

   Promoter reporter

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
   

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJW1948

   Plasmid

   #154340

   Purpose

   Enhancer reporter

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR
   

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

   Plasmid

   #136656

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9:MTH17CL396 Chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and hU6

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. B-HypaSpCas9

   Plasmid

   #126763

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A; M694A; Q695A; H698A; amino acids 1005-1013…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-FLAG-B-eSpCas9

   Plasmid

   #126772

   Purpose

   Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6_sgRNA_CAG_hSt1Cas9_TH1477

   Plasmid

   #136655

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9:TH1477 Chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and hU6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid

    #136653

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and hU6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ARB366

    Plasmid

    #124049

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. ARB367

    Plasmid

    #124050

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTone-gata2aECE-nsGFP

    Plasmid

    #132975

    Purpose

    gata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backbone

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Inserts

    nuclear localized sfGFP
    gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
    

    Use

    Unspecified; Transposon-mediated

    Tags

    6x myc and SV40 NLS

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW2694_pCAG-PV1-pMag-L1-iCre-252C-BGHpA

    Plasmid

    #108820

    Purpose

    Expresses aa252 to C-terminus of Cre recombinase fused to pMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa252 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and pMag

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpA

    Plasmid

    #108822

    Purpose

    Expresses aa257 to C-terminus of Cre recombinase fused to pMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa257 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and pMag

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpA

    Plasmid

    #108800

    Purpose

    Expresses aa397 to C-terminus of PhiC31 integrase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa397 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpA

    Plasmid

    #108804

    Purpose

    Expresses aa572 to C-terminus of PhiC31 integrase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa572 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(-)-HF2-BE3

    Plasmid

    #120397

    Purpose

    Expresses HF2-BE3 in mammalian cells

    Depositor

    Junjiu Huang

    Article

    Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

    Insert

    HF2-BE3
    

    Expression

    Mammalian

    Mutation

    Mammalian codon-optimized Cas9-HF

    Promoter

    CMV

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTE3314

    Plasmid

    #107530

    Purpose

    Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hFnCpf1(RVR)
    

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N607R, K613V, N617R

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTE3319

    Plasmid

    #107533

    Purpose

    Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(RR)
    

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K637R

    Promoter

    CMV

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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