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pEGFP-C1-hRabin8 L196A

Plasmid

#26727

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

L196A: GEF domain mutant.

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
pRS416-Aga1p-FAPB2.3.6L1TAG

Plasmid

#158148

Purpose

Yeast display reporter for non-display yeast strains (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Expression

Yeast

Mutation

Cloned in a TAG codon at the first position of th…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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SBP-mCherry-Ecadherin_puromycin

Plasmid

#65293

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_flGalT-SBP-tagBFP

Plasmid

#65274

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-deltaR8.2(A14C,E45C,A92E)

Plasmid

#79048

Purpose

mammalian expression, lentiviral packaging

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-HT/prsA

Plasmid

#73624

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MT21-Ubc9

Plasmid

#17273

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2006 Jan . 80(1):342-52.

Insert

Ubc9 (Ube2i Mouse)

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-PIG-miR-30b

Plasmid

#64232

Purpose

Expresses miR-30b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-30b (MIR30B Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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wt1aCreERT2

Plasmid

#108464

Purpose

Used to generate a cryst:mCherry wt1a:CreERT2 zebrafish transgenic line.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

wt1aCreERT2

Use

Cre/Lox

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG TBC1D13

Plasmid

#87953

Purpose

Expresses flag tagged mouse TBC1D13

Depositor

David James

Article

Davey et al Traffic. 2012 Oct;13(10):1429-41. doi: 1

Insert

TBC1D13 (Tbc1d13 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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RHEB

Plasmid

#55683

Purpose

expression of mouse RHEB

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RHEB (Rheb Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pMR-A-4

Plasmid

#138518

Purpose

P3b

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG Enthoprotin Full Length

Plasmid

#47419

Depositor

Peter McPherson

Article

Wasiak et al J Cell Biol. 2002 Sep 2;158(5):855-62. d

Insert

Enthoprotin

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pBT138b-proA

Plasmid

#138514

Purpose

P3g

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCLX-UBI-Tred

Plasmid

#27246

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

TurboRFP

Use

Lentiviral

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pVH016

Plasmid

#80400

Purpose

No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

ffGFP-3xFLAG
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

C-terminal 3xFLAG tag and C-terminal LZx domain

Expression

Bacterial

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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RAP2A

Plasmid

#55666

Purpose

expression of mouse RAP2A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RAP2A (Rap2a Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pAXY0016

Plasmid

#205585

Purpose

p3flag_HygR(1-89)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

HygR-NpuDnaE(N)_homo-codon-OP

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDY-HA1-HA2-neo

Plasmid

#109123

Purpose

FR cassette provider to introduce FR cassette to a BAC by recombineering

Depositor

David Gilbert

Article

Sima et al Nucleic Acids Res. 2018 Feb 28;46(4):181

Inserts

HA1
HA2

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-4EHP-mISG15

Plasmid

#17355

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP-mISG15 (EIF4E2 Mouse, Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only