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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,179 results for: CRISPR-Cas9

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Showing: 4761 - 4780 of 14179 results

  1. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

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  2. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Article
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available Since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    60820-plasmid-map-sequence-id-371636

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  4. pMJ923

    Plasmid
    #78313
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-mCherry-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78313-plasmid-map-sequence-id-148391

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  5. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid
    #99692
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    99692-plasmid-map-sequence-id-194594

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  10. pGMC00022 (aka pRC0433)

    Plasmid
    #195321
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with hygromycin selection
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195321-plasmid-map-sequence-id-385749

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  11. pGMC00020 (aka pRC0431)

    Plasmid
    #195304
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with blasticidin selection
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195304-plasmid-map-sequence-id-385590

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  12. pGMC00021 (aka pRC0432)

    Plasmid
    #195305
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with puromycin selection
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195305-plasmid-map-sequence-id-385584

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  20. psBIG1a

    Plasmid
    #229957
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229957-plasmid-map-sequence-id-461574

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Showing: 4761 - 4780 of 14179 results