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dCas9-miniTurbo

Plasmid

#219822

Purpose

EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

Depositor

Philipp Maass

Insert

dCas9

Use

CRISPR

Tags

miniTurbo

Expression

Mammalian

Mutation

D10A/H841A

Promoter

EF1a

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG_Xph20-mRuby2-CCR5TC

Plasmid

#135531

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Publication

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

mRuby2

Expression

Mammalian

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SunTagng14aa

Plasmid

#106439

Purpose

Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

Depositor

Steven Jacobsen

Publication

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)

Plasmid

#73477

Purpose

Encodes the G protein to enable transsynaptic RAV infection

Depositor

Thomas Jessell

Publication

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

H2B-3XHA-P2A-N2c(G)

Use

AAV

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV/MASC_(GLuc)_TOP1

Plasmid

#68439

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSA120

Plasmid

#59789

Purpose

C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon

Depositor

Jeremy Nance

Publication

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

hsp-16.41 promoter
zif-1 coding
gpd-2/gpd-3 intergenic sequence
mCherry (worm)
unc-54 3' UTR

Expression

Bacterial

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVdG-1mOrange2-5PSD95EGFP

Plasmid

#52482

Purpose

Expresses mOrange2 and PSD-95-EGFP

Depositor

Sebastian Seung

Publication

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mOrange2
PSD-95-EGFP

Use

Rabies virus

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBZCas13b-HEPN

Plasmid

#89901

Purpose

Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.

Depositor

Feng Zhang

Publication

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

Cas13b

Expression

Bacterial

Mutation

R116A/H121A/R1177A/H1182A

Promoter

Lac

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTcI38-Wasabi

Plasmid

#86117

Purpose

mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38

Depositor

Mikhail Shapiro

Publication

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TcI38
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A67T, M1V, L65S, K68R, F115L, D126G, D188G

Promoter

pR/pL and pR/pL, LacI

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ1201

Plasmid

#44483

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, peel-1, MCS

Use

Multiple cloning site vector

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-Ago4

Plasmid

#21536

Depositor

Edward Chan

Publication

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago4 (AGO4 Human)

Tags

GFP

Expression

Mammalian

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSV2 neuT

Plasmid

#10919

Depositor

Bob Weinberg

Publication

Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

Insert

neuT (Erbb2 Rat)

Expression

Mammalian

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
SpCas9-HF1

Plasmid

#138556

Purpose

Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

SpCas9-HF1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pNeoXTR f0

Plasmid

#69157

Purpose

mouse targeting vector

Depositor

David Feldser

Publication

Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

Inserts

DTA
NeoR
GFP
AmpR

Use

Mouse Targeting

Promoter

Amp promoter, PGK, and none

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAWp9

Plasmid

#73851

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}

Depositor

Timothy Lu

Publication

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}

Use

Lentiviral

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJ2072.2_CRISPRlator

Plasmid

#140674

Purpose

CRISPRlator

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

CRISPRlator

Use

Synthetic Biology

Expression

Bacterial

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141C

Plasmid

#69292

Purpose

Gateway entry vector; single gRNA under OsU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

Plasmid

#224435

Purpose

AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Publication

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mScarlet-KASH
U6-gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-RAB-AKARev

Plasmid

#118478

Purpose

ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

Plasmid

#86418

Purpose

ONM/ER split GFP1-10

Depositor

Sue Jaspersen

Publication

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP1-10-Scs2TM

Tags

GFP1-10

Expression

Yeast

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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