We narrowed to 6,250 results for: nls

1. pTE3316

   Plasmid

   #107532

   Purpose

   Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(RR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N607R, K671R

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE3317

   Plasmid

   #107531

   Purpose

   Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(RVR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N576R, K582V, N586R

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPC-Flag-CHES1L-dC

   Plasmid

   #51839

   Purpose

   Flag-CHES1L mutant lacking the C-terminal and NLS domains

   Depositor

   Gerardo Ferbeyre

   Article

   Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

   Insert

   CHES1 (FOXN3 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Mutation

   contains CHES1 amino acids 1-205

   Available Since

   Aug. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

4. FNE2-A72

   Plasmid

   #44608

   Depositor

   Robert Oshima

   Article

   Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

   Insert

   Ets2-A72 (Ets2 Mouse)
   

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   THR-72 converted to Alanine

   Promoter

   CMV

   Available Since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pEMS1241

   Plasmid

   #29123

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple12
   

   Tags

   EGFP-NLS

   Available Since

   Nov. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS1207

   Plasmid

   #29104

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple235
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Available Since

   Oct. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA428

   Plasmid

   #216055

   Purpose

   Fragmid fragment: (C' terminus) NpNLS; 3xHA; c-mycNLS; c-mycNLS

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   NpNLS_v1.1; 3xHA_v1.1; c-mycNLS_v1.2; c-mycNLS_v1.3
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+ heiCas9

   Plasmid

   #186929

   Purpose

   For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

   Depositor

   Joachim Wittbrodt

   Article

   Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

   Insert

   myc-flexible linker-oNLS-Cas9-oNLS
   

   Use

   CRISPR

   Tags

   myc-flexible linker-optimizedNLS and optimizedNLS

   Expression

   Mammalian

   Promoter

   sp6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-dCpf1-eBE

   Plasmid

   #107688

   Purpose

   Expresses dLbCpf1-eBE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-eBE
   

   Expression

   Mammalian

   Mutation

   dLbCpf1(D832A/E925A/D1148A)

   Promoter

   CMV

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PEmax

    Plasmid

    #174820

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-PEmax for IVT

    Plasmid

    #178113

    Purpose

    Template for in vitro transcription of PEmax

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PEmax-P2A-GFP

    Plasmid

    #180020

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-EGFP
    

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PEmax-P2A-BSD

    Plasmid

    #174821

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-BSD
    

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PEmax-P2A-hMLH1dn

    Plasmid

    #174828

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-hMLH1dn
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-fDIO-Cre (AAV1)

    Viral Prep

    #121675-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Esteban Engel, Alexander Nectow

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-EF1a-fDIO-Cre (AAV8)

    Viral Prep

    #121675-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Esteban Engel, Alexander Nectow

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-EF1a-fDIO-Cre (AAV9)

    Viral Prep

    #121675-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Esteban Engel, Alexander Nectow

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-EF1a-fDIO-Cre (AAV5)

    Viral Prep

    #121675-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Esteban Engel, Alexander Nectow

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

19. L4038

    Plasmid

    #1620

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    let858enh>>, let858enh<<, let858-455,…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. L4054

    Plasmid

    #1632

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    IVSA, SV40NLS, SV40NLS, SV40NLS, SV40NLS, gfpN, I…

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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