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Plasmid#234810PurposeExpress Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1DepositorUseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEEK promoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only
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Phage-PGK-Flag-HA-FKBP-T(G177D)
Plasmid#181735Purposefor lentiviral expression of brachyury ORF in mammalian cellsDepositorInsertT (brachyury)
UseLentiviralTagsExpressionMutationa silent mutation in the PAM site of an sgRNAt ta…PromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Venus-Bad-2A-pEGFP-C1
Plasmid#166748PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domainDepositorInsertBad-2A (BAD Human)
UseTagsVenusExpressionMammalianMutation2 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPC-JAG1-mVenus
Plasmid#171170PurposeRetroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).DepositorInsertJAG1 (JAG1 Human)
UseRetroviralTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLPC-dnMAML1-mVenus
Plasmid#171023PurposeRetroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).DepositorInsertMAML1 Dominant Negative (MAML1 Human)
UseRetroviralTagsmVenusExpressionMammalianMutationamino acids 12-74 only, hence conferring dominant…PromoterCMVAvailable sinceApril 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR
Plasmid#181974PurposeExpresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.DepositorInsertFMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
UseLentiviralTagsExpressionMutationNGFR truncation: aa 11-276PromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR
Plasmid#181975PurposeExpresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.DepositorInsertFMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
UseLentiviralTagsExpressionMutationNGFR truncation: aa 11-276PromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastbac-Flag-BRCA1
Plasmid#223228PurposeInsect expression construct for Flag-tagged BRCA1 protein production and purificationDepositorInsertBreast Cancer 1 (BRCA1 Human)
UseTagsFlagExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-DIO-mCherry-nega miR
Plasmid#137732PurposeLentivirus vector for control miR in a Cre-dependent mannerDepositorInsertcontrol microRNA
UseLentiviralTagsmChherryExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL-4E-pEGFP-C1
Plasmid#166742PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, BimEL with mutation in BH3 domain to disrupt binding anti-apoptotic proteinsDepositorInsertBimEL-4E (Bcl2l11 Mouse)
UseTagsVenusExpressionMammalianMutation4 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimL-4E-pEGFP-C1
Plasmid#166741PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, BimL with mutation in BH3 domain to disrupt binding anti-apoptotic proteinsDepositorInsertBimL-4E (Bcl2l11 Mouse)
UseTagsVenusExpressionMammalianMutation4 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL-N1ICD-Flag
Plasmid#171171PurposeRetroviral vector for expression of NOTCH1 intracellular domain with a C-terminal FLAG tag (to be used in conjunction with Phoenix packaging cells).DepositorInsertNOTCH1 intracellular domain (NOTCH1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationWill express residues 1758-2556 of human NOTCH1, …PromoterViral LTRAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605
Plasmid#108362PurposeExpression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegansDepositorInsertsUseTags2xFlag, MYC, T2A peptide, and TY1 linkerExpressionWormMutationPromoterA rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…Available sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-DIO-mCherry-TLR4miR-TLR2miR
Plasmid#137733PurposeLentivirus vector for TLR2/4 miR in a Cre-dependent mannerDepositorInsertTKR2/4 microRNA
UseLentiviralTagsmChherryExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Bad)-pEGFP-C1
Plasmid#166758PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of BadDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Bad …PromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-CPI-17-T38E
Plasmid#100773PurposeMammalian expression of CPI-17 phospho-mimeticDepositorInsertCPI-17-T38E (PPP1R14A Human)
UseTagsHAExpressionMammalianMutationT38EPromoterCMVAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-CPI-17-T38E-NLS
Plasmid#100778PurposeMammalian expression of CPI-17 phospho-mimetic nuclearDepositorInsertCPI-17-T38E-NLS (PPP1R14A Human)
UseTagsHAExpressionMammalianMutationT38EPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-CPI-17-T38E-NES
Plasmid#100777PurposeMammalian expression of CPI-17 phospho-mimetic cytosolicDepositorInsertCPI-17-T38E-NES (PPP1R14A Human)
UseTagsHAExpressionMammalianMutationT38EPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-T38E-NLS
Plasmid#100790PurposeMammalian expression of Fluorescent CPI-17 phospho mimetic nuclearDepositorInsertCPI-17-T38E-NLS (PPP1R14A Human)
UseTagsDsRedExpressionMammalianMutationT38EPromoterCMVAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-T38E-NES
Plasmid#100789PurposeMammalian expression of Fluorescent CPI-17 phospho mimetic cytosolicDepositorInsertCPI-17-T38E-NES (PPP1R14A Human)
UseTagsDsRedExpressionMammalianMutationT38EPromoterCMVAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only