We narrowed to 4,484 results for: WOL

1. PGL4-4X Mut Renilla

   Plasmid

   #70040

   Purpose

   Control plasmid for testing 5'UTR folding elements

   Depositor

   Hans-Guido Wendel

   Article

   Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

   Insert

   Random sequence matched for GC-content
   

   Use

   Luciferase

   Tags

   renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLKO.1-puro U6 sgRNA Oct4A -158

   Plasmid

   #50922

   Purpose

   U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   Oct4A -158 sgRNA (POU5F1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p11d-tRPA-32Asp8

   Plasmid

   #102617

   Purpose

   Expression of human RPA with aspartic acid substituted phosphorylation domain

   Depositor

   Marc Wold

   Article

   Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-9

   Inserts

   RPA1 (RPA1 Human)
   RPA3 (RPA3 )
   RPA2 (RPA2 )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   with aspartic acid substituted phosphorylation do…

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PGL4-4X TE Down (GQs) Renilla

   Plasmid

   #70039

   Purpose

   Plasmid for testing 5’UTR TE Down (G-quadruplex) folding elements

   Depositor

   Hans-Guido Wendel

   Article

   Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

   Insert

   4x TE Down (G-quadruplexes) in 5'UTR
   

   Use

   Luciferase

   Tags

   renilla luciferase

   Expression

   Mammalian

   Mutation

   4 repeats of TE down motif (G-quadruplexes) in 5&…

   Promoter

   SV40

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

5. MIG-eIF4A1 P159E

   Plasmid

   #70042

   Purpose

   Expression of eIF4A1 P159E mutant construct in mammalian cells.

   Depositor

   Hans-Guido Wendel

   Article

   Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

   Insert

   eIF4A1 (Eif4a1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   P159E

   Promoter

   CMV

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. MIG-eIF4A1 F163L

   Plasmid

   #70043

   Purpose

   Expression of eIF4A1 F163L mutant in mammalian cells

   Depositor

   Hans-Guido Wendel

   Article

   Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

   Insert

   eIF4A1 (Eif4a1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   F163L

   Promoter

   CMV

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. MIG-eIF4A1 Q195E

   Plasmid

   #70044

   Purpose

   Expression of eIF4A1 Q195E mutant in mammalian cells

   Depositor

   Hans-Guido Wendel

   Article

   Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

   Insert

   eIF4A1 (Eif4a1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Q195E

   Promoter

   CMV

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

   Plasmid

   #69219

   Purpose

   Expresses wild type SpCas9 fused to Zif268 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   Zif268 (Egr1 Mouse)
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-TAC-OSKM

   Plasmid

   #80481

   Purpose

   piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PB-TAC-MKOS

    Plasmid

    #80484

    Purpose

    piggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    MKOS (Myc, Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB-TET-MKOS

    Plasmid

    #20959

    Purpose

    piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB-TAC-OKMS

    Plasmid

    #80480

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB-TAC-OK+9MS

    Plasmid

    #80482

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-TAC-EB-C5

    Plasmid

    #80485

    Purpose

    piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    lin-28 homolog A (C. elegans) (Pou5f1, Sox2, Klf4, Myc, Lin28a Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Human Transporter Knockout library

    Pooled Library

    #213695

    Purpose

    Next generation transporter-focused CRISPR/Cas9 library targeting transmembrane transporters to test the impact of transporters on biological processes.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Ferdigg et al Life Sci Alliance. 2024 Nov 22;8(2):e202

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pJDS78-GG-SNG

    Plasmid

    #44716

    Depositor

    Nathan Lawson

    Article

    Gupta et al Genome Res. 2013 Mar 11.

    Type

    Empty backbone

    Use

    Tags

    3X Flag and FOKI WT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJDS74-GG-NNN

    Plasmid

    #44715

    Depositor

    Nathan Lawson

    Article

    Gupta et al Genome Res. 2013 Mar 11.

    Type

    Empty backbone

    Use

    Tags

    3X Flag and FOKI WT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJDS70-GG-SNI

    Plasmid

    #44713

    Depositor

    Nathan Lawson

    Article

    Gupta et al Genome Res. 2013 Mar 11.

    Type

    Empty backbone

    Use

    Tags

    3X Flag and FOKI WT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJDS71-GG-SHD

    Plasmid

    #44714

    Depositor

    Nathan Lawson

    Article

    Gupta et al Genome Res. 2013 Mar 11.

    Type

    Empty backbone

    Use

    Tags

    3X Flag and FOKI WT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. plp

    Plasmid

    #22310

    Depositor

    David Bartel

    Article

    Drinnenberg et al Science. 2009 Oct 23;326(5952):544-550.

    Type

    Empty backbone

    Use

    Integrating plasmid

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge