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Showing: 461 - 480 of 3297 results

  2. pJB50

    Plasmid
    #226311
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-7
    Use
    Tags
    Expression
    Bacterial
    Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226311-plasmid-map-sequence-id-449923

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  4. pUDP044

    Plasmid
    #101168
    Purpose
    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101168-plasmid-map-sequence-id-199309

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  8. pUDP123

    Plasmid
    #107269
    Purpose
    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    107269-plasmid-map-sequence-id-208617

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  11. pDrop

    Plasmid
    #184872
    Purpose
    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    lacZalpha,ccdB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    184872-plasmid-map-sequence-id-364202

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  19. pCRIS

    Plasmid
    #120424
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3, IS150
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    120424-plasmid-map-sequence-id-233246

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Showing: 461 - 480 of 3297 results