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pLV_iRFP_P2AT2A_mScarlet-I_Myl9

Plasmid

#172474

Purpose

Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.

Depositor

Sean R Collins

Article

Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:

Inserts

iRFP670
mScarlet-I-Myl9 (MYL9 Human)

Use

Lentiviral

Tags

mScarlet-I

Expression

Mammalian

Promoter

EF1alpha (with UCOE) and none (same transcript as…

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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StrKDEL-IRES_mScarleti_Sec23A

Plasmid

#117273

Purpose

Expresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23A

Depositor

David Stephens

Article

McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

Inserts

SEC23A (SEC23A Human)
Streptavidin-KDEL

Tags

mScarlet-i

Expression

Mammalian

Mutation

Human Sec23A; Sequencing showed a mutation in Sec…

Promoter

CMV and IRES

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

Plasmid

#178087

Purpose

HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

mScarlet-I mTOR signaling indicator (TOSI)

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCarCBADE_ProB(I69E)A

Plasmid

#117756

Purpose

ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2_mScarletI_mVenusNB-MrkHR113A

Plasmid

#182292

Purpose

expresses c-di-GMP mutant biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkHR113A

Tags

mVenusNB internal fusion to MrkH with a R113A poi…

Expression

Bacterial

Mutation

changed arginine 113 to alanine, MrkH numbering …

Promoter

proC

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-CARD11

Plasmid

#23482

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CARD11 (CARD11 Human)

Use

Gateway donor vector

Mutation

A291C,C292T,1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLifeAct_mScarlet-H_N1

Plasmid

#85055

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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mCardinal-H2B-C-10

Plasmid

#56162

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H2B (H2BC11 Human)

Tags

mCardinal

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLARRYv2-mScarlet

Plasmid

#233210

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with mScarlet marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

Plasmid

#110623

Purpose

It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH mScarlet-I- IRES- sYFP2 PH

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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M12 pCarGFP2

Plasmid

#17149

Depositor

Randall Moon

Insert

cardiac actin promoter

Use

Xenopus expression

Tags

GFP

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pOVC1_TtCarH-CBD_X

Plasmid

#131179

Purpose

Expresses TtCarH-CBD (Thermus termophilus) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

TtCarH-CBD

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_MxCarH-CBD_X

Plasmid

#131178

Purpose

Expresses MxCarH-CBD (Myxococcus xanthus) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

MxCarH-CBD

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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mCardinal-Tubulin-C-18

Plasmid

#56176

Purpose

Localization: Microtubules, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Tubulin (TUBA1B Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCytERM_mScarlet-H_N1

Plasmid

#85067

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

mScarlet-H

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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mCardinal-PDHA1-N-10

Plasmid

#56172

Purpose

Localization: Mitochondrial Matrix, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

PDHA1 (PDHA1 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-LaminB1-10

Plasmid

#56165

Purpose

Localization: Nuclear Envelope, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

LaminB1 (LMNB1 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pmScarlet-H_alphaTubulin_C1

Plasmid

#85046

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART2

Plasmid

#133250

Purpose

Express FLAG-tagged MCART2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART2 (SLC25A52 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Km ymScarletI

Plasmid

#193960

Purpose

TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeast

Depositor

Bas Teusink

Insert

ymScarletI

Expression

Yeast

Promoter

TEF1

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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