Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,594 results for: gem

Clear keywords
Showing: 461 - 480 of 2594 results

  1. pGem-LP1-loxP-bar-lox2272-71

    Plasmid
    #168785
    Purpose
    Vector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Inserts
    bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    loxP
    lox2272-71
    Use
    Cre/Lox and Synthetic Biology
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168785-plasmid-map-sequence-id-350000

    Enlarge

  2. pGem-LP2-loxP-bar-lox2272-71

    Plasmid
    #168786
    Purpose
    Vector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Inserts
    bar
    IS1 Homology arm 1
    IS1 Homology arm 2
    loxP
    lox2272-71
    Use
    Cre/Lox and Synthetic Biology
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168786-plasmid-map-sequence-id-353782

    Enlarge

  5. pGEM-myr-Citrine-SV40pA-FKF

    Plasmid
    #119865
    Purpose
    Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    2x myristoylation
    Expression
    Bacterial
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119865-plasmid-map-sequence-id-239419

    Enlarge

  6. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid
    #71707
    Purpose
    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid
    Depositor
    Giulio Draetta
    Article
    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16
    Insert
    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    Use
    CRISPR
    Tags
    3xFLAG and hGEM(1/110)
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    71707-plasmid-map-sequence-id-424083

    Enlarge

  12. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid
    #119864
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    HA-tagged biotin ligase (BirA), Thosea asigna vir…
    Expression
    Bacterial
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119864-plasmid-map-sequence-id-239418

    Enlarge

  13. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid
    #140200
    Purpose
    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    dLbCas12a crRNA scaffold
    Promoter
    PgpdA
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140200-plasmid-map-sequence-id-310179

    Enlarge

  14. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid
    #140204
    Purpose
    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Step 1 of cloning c…
    Promoter
    Aspergillus fumigatus U3 (RNAPIII).
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    140204-plasmid-map-sequence-id-309884

    Enlarge

  15. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79889-plasmid-map-sequence-id-150065

    Enlarge

  16. pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)

    Plasmid
    #225443
    Purpose
    Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.
    Depositor
    Diego Orzaez
    Article
    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1
    Insert
    BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225443-plasmid-map-sequence-id-447736

    Enlarge

  20. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid
    #140196
    Purpose
    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta) , NLS
    Promoter
    PgpdA and PtrpC
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140196-plasmid-map-sequence-id-309706

    Enlarge

Showing: 461 - 480 of 2594 results