We narrowed to 147,612 results for: ins;

1. Proinsulin-NanoLuc in pLX304

   Plasmid

   #62057

   Purpose

   Luminescent reporter of insulin secretion

   Depositor

   David Altshuler

   Article

   Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

   Insert

   Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Inserted NanoLuc into C-peptide of mouse Ins2 cod…

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-INSR-V5/HIS

   Plasmid

   #201980

   Purpose

   expression of human INSR receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   INSR receptor tyrosine kinase (INSR Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBluescriptII[attB/Ins1]

   Plasmid

   #74100

   Purpose

   "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Phi C31 attb element
   Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABEpuro-ERBB2 A775_G776insYVMA

   Plasmid

   #40982

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A775_G776insYVMA

   Promoter

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXT128_TwinStrep-SUMO-ShCas12k

   Plasmid

   #135524

   Purpose

   Protein expression construct for ShCast12k

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShCas12k
   

   Use

   Tags

   TwinStrep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-EGFR-H773_V774insNPH

   Plasmid

   #116262

   Purpose

   Lentiviral expression of EGFR H773_V774insNPH

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   H773_V774insNPH

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-EGFR-A763_Y764insFQEA

   Plasmid

   #116233

   Purpose

   Lentiviral expression of EGFR A763_Y764insFQEA

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   A763_Y764insFQEA

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXT130_TwinStrep-SUMO-ShTnsC

   Plasmid

   #135526

   Purpose

   Protein expression construct for ShTnsC

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsC
   

   Use

   Tags

   TwinStrep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXT131_TwinStrep-SUMO-ShTniQ

   Plasmid

   #135527

   Purpose

   Protein expression construct for ShTniQ

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTniQ
   

   Use

   Tags

   TwinStrep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXT129_TwinStrep-SUMO-ShTnsB

    Plasmid

    #135525

    Purpose

    Protein expression construct for ShTnsB

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-EGFR-M766_A767insAI

    Plasmid

    #116279

    Purpose

    Lentiviral expression of EGFR M766_A767insAI

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    M766_A767insAI

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

    Plasmid

    #232344

    Purpose

    Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

    Depositor

    Philip Wong

    Article

    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

    Insert

    HDGF Like 2 (HDGFL2 Human)
    

    Use

    Tags

    GGGS linker, 6xHis and Twin-strep, TEV protease s…

    Expression

    Mammalian

    Mutation

    TDP-43 regulated cryptic exon

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-CMV-HDGFL2_WT_TwinstrepTEV_6xHis

    Plasmid

    #232343

    Purpose

    Expresses wild-type human HDGFL2 protein with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

    Depositor

    Philip Wong

    Article

    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

    Insert

    HDGF like 2 (HDGFL2 Human)
    

    Use

    Tags

    GGGS linker, 6xHis and Twin-strep, TEV protease s…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEx-insertion-erm

    Plasmid

    #172210

    Purpose

    For genetic insertion in erythromycin-sensitive Prevotella copri strains

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Genetic insertion in prevotella copri

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. INS-2A-luciferase-2A-tdT

    Plasmid

    #159348

    Purpose

    Human INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    Luciferase-2A-tdTomato
    

    Use

    Ins locus knockin donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid

    #197264

    Purpose

    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Type

    Empty backbone

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTMEM-PhoCl-Insert

    Plasmid

    #219674

    Purpose

    Caging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarlet

    Depositor

    Helge Ewers

    Article

    Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

    Insert

    TMEM-PhoCl-rtTA3
    

    Use

    Tags

    PhoCl

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pcDNA3.1-ccdB-TwinStrep

    Plasmid

    #214988

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-EGFR-Y764_V765insHH

    Plasmid

    #116319

    Purpose

    Lentiviral expression of EGFR Y764_V765insHH

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Y764_V765insHH

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRosa26-4xInsulator (JP6)

    Plasmid

    #99627

    Purpose

    IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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