We narrowed to 150,016 results for: ins;

1. pJBL-RNAINS3

   Plasmid

   #69455

   Purpose

   RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

   Depositor

   Julius Lucks

   Article

   Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

   Insert

   RNA-IN S3
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   mutations for S3 variant (from wt)

   Promoter

   J23119 and J23119 (sigma 70)

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tg-UBC (TgTwinScan_2218)

   Plasmid

   #25565

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Tg-UBC (TgTwinScan_2218)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

   Plasmid

   #195048

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

   Plasmid

   #195047

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

   Plasmid

   #195046

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Expression

   Yeast

   Promoter

   pADH2, pGPD, and pXYL

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Annexin A7-TEV-TwinStrep

   Plasmid

   #198635

   Purpose

   Annexin A7 fibrillization and lipid binding

   Depositor

   Lalit Deshmukh

   Article

   Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

   Insert

   Annexin A7 (ANXA7 Human)
   

   Tags

   TEV-GS-Dual Strep

   Expression

   Bacterial

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TwinStrepSUMO-pvc10-Cas9

   Plasmid

   #243755

   Purpose

   For affinity purification of Pvc10 fused to Cas9

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

   Insert

   pvc10-Cas9
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. TwinStrepSUMO-Ad5-SpyCatcher

   Plasmid

   #243751

   Purpose

   For affinity purification of A5 knob fused to SpyCatcher

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

   Insert

   Ad5-SpyCatcher
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABEpuro-ERBB2 A775_G776insYVMA

   Plasmid

   #40982

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   A775_G776insYVMA

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Proinsulin-NanoLuc in pLX304

    Plasmid

    #62057

    Purpose

    Luminescent reporter of insulin secretion

    Depositor

    David Altshuler

    Article

    Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

    Insert

    Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Mutation

    Inserted NanoLuc into C-peptide of mouse Ins2 cod…

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-EGFR-E746_T751delinsA

    Plasmid

    #116246

    Purpose

    Lentiviral expression of EGFR E746_T751delinsA

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Mutation

    E746_T751delinsA

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-INSR-V5/HIS

    Plasmid

    #201980

    Purpose

    expression of human INSR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    INSR receptor tyrosine kinase (INSR Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

    Plasmid

    #232344

    Purpose

    Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

    Depositor

    Philip Wong

    Article

    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

    Insert

    HDGF Like 2 (HDGFL2 Human)
    

    Tags

    GGGS linker, 6xHis and Twin-strep, TEV protease s…

    Expression

    Mammalian

    Mutation

    TDP-43 regulated cryptic exon

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTwist-CMV-HDGFL2_WT_TwinstrepTEV_6xHis

    Plasmid

    #232343

    Purpose

    Expresses wild-type human HDGFL2 protein with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

    Depositor

    Philip Wong

    Article

    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

    Insert

    HDGF like 2 (HDGFL2 Human)
    

    Tags

    GGGS linker, 6xHis and Twin-strep, TEV protease s…

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD24-ALFA-TwinStrep

    Plasmid

    #240248

    Purpose

    Arabinose-inducible plasmid with ALFA and Twin-Strep tags

    Depositor

    Krishna Reddy, Olga Boudker

    Article

    Reddy et al Nat Struct Mol Biol (2025).

    Type

    Empty backbone

    Tags

    10x HIS, ALFA, Thrombin, and Twin-Strep

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXT128_TwinStrep-SUMO-ShCas12k

    Plasmid

    #135524

    Purpose

    Protein expression construct for ShCast12k

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShCas12k
    

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBluescriptII[attB/Ins1]

    Plasmid

    #74100

    Purpose

    "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Phi C31 attb element
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
    

    Expression

    Bacterial

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXT130_TwinStrep-SUMO-ShTnsC

    Plasmid

    #135526

    Purpose

    Protein expression construct for ShTnsC

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsC
    

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXT131_TwinStrep-SUMO-ShTniQ

    Plasmid

    #135527

    Purpose

    Protein expression construct for ShTniQ

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTniQ
    

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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