We narrowed to 2,314 results for: tub

1. pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

   Plasmid

   #154303

   Purpose

   Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

   Depositor

   Shigehiko Yumura

   Article

   Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

   Inserts

   histone H1
   γ-tubulin
   

   Use

   Dictyostelium expression

   Tags

   mEGFP and mRuby3

   Expression

   Mutation

   Promoter

   actin15

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINK-OTUB2 (full-length, aa 1-234)

   Plasmid

   #61421

   Purpose

   Expresses human OTUB2 (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTER GAMMA-TUBULIN shRNA Human

   Plasmid

   #87955

   Purpose

   reduced the expression of gamma-tubulin in human cells

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   sh gamma-tubulin (TUBG1 Human)
   

   Use

   Tags

   No-tag

   Expression

   Mammalian

   Mutation

   The protein is a sh-gamma-tubulin resistant gene.…

   Promoter

   Available since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-OTUB1 T134R,C91S-pcDNA3.1

   Plasmid

   #118212

   Purpose

   Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed cysteine 91 to serine and threonine 134 t…

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-mCerulean3-TUBB1A (JDW 1508)

   Plasmid

   #242555

   Purpose

   Gateway middle entry clone containing mCerulean3-TUBB1A; Cyan fluorescent reporter for visualizing tubulin in cells

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mCerulean3-TUBB1A
   

   Use

   Gateway subcloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTub-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199210

   Purpose

   For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   split-intein Gal4[1-20]-N-int
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. L1B42-pGATA23-Bxb1-SAUR-tUBQ10

   Plasmid

   #219705

   Purpose

   Level 1 pGATA23-Bxb1-SAUR-tUBQ10, for lateral root specific expression of Bxb1 integrase with SAUR tag

   Depositor

   Jennifer Nemhauser

   Insert

   pGATA23:Bxb1:SAUR:tUBQ10
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-mCerulean3-TUBB1A (JDW 1367)

   Plasmid

   #224503

   Purpose

   Gateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cells

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mCerulean3-TUBB1A
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. L0-T12 GG2-ER-tagBFP-tUBQ10-GG3

   Plasmid

   #219695

   Purpose

   Level 0 to construct history-dependent target

   Depositor

   Jennifer Nemhauser

   Insert

   GG2-ER-tagBFP-tUBQ10-GG3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. L1P137 - pAHP6-PhiC31-NLS-tUBQ1

    Plasmid

    #219702

    Purpose

    Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase

    Depositor

    Jennifer Nemhauser

    Insert

    pAHP6:PhiC31-NLS:tUBQ1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-EGFP-C1-tubgenomic-PTC62-deltaIntron3_D

    Plasmid

    #146146

    Purpose

    Mammalian Expression of tubgenomic-PTC62-delIntron3

    Depositor

    Elisa Izaurralde

    Article

    Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

    Insert

    tubgenomic-PTC62-delIntron3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACEBac1-gamma-tubulin-TEV-His6

    Plasmid

    #178067

    Purpose

    Insect cell expression of gamma-tubulin-TEV-His6

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    gamma-tubulin (TUBG1 Human)
    

    Use

    Tags

    TEV-His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKSB- PUb-GFPTub56D GGGG AAGA

    Plasmid

    #174537

    Purpose

    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes, with DmTub56D terminator

    Depositor

    Eric Marois

    Insert

    PUb-GFP-Tub56Dterminator BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

    Plasmid

    #87259

    Purpose

    YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133

    Depositor

    David Sibley

    Article

    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

    Inserts

    YFP-mAID-3HA
    HXGPRT
    

    Use

    Toxoplasma expression

    Tags

    3HA and mAID

    Expression

    Mutation

    Codon optimized for Toxoplasma gondii expression

    Promoter

    TgDHFR-TS 463 bp and TgTUB1 378 bp

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

    Plasmid

    #211840

    Purpose

    Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA and mCherry

    Expression

    Plant

    Mutation

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

    Plasmid

    #131508

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

    Plasmid

    #211843

    Purpose

    Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA and mCherry

    Expression

    Plant

    Mutation

    C492A

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid

    #171090

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

    Expression

    Mutation

    D9A, H599A

    Promoter

    TUB1 (Toxoplasma gondii), transcriptionally linke…

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT

    Plasmid

    #87260

    Purpose

    YFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.

    Depositor

    David Sibley

    Article

    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

    Inserts

    YFP-AID-3HA
    HXGPRT
    

    Use

    Toxoplasma expression

    Tags

    3HA and AID

    Expression

    Mutation

    Promoter

    TgDHFR-TS 463 bp and TgTUB1 378 bp

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

    Plasmid

    #211839

    Purpose

    Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Mutation

    R181A R185A

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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