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pBL119-2

Plasmid

#104336

Purpose

Expresses thsR from a TetR regulated promoter under ATC control. Expresses sfGFP from a thsR regulated promoter. Expresses mCherry from a constituative promoter.

Depositor

Jeffrey Tabor

Article

Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

Insert

Thiosulfate response regulator

Expression

Bacterial

Promoter

PLTetO1

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S669A

Plasmid

#19282

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc S669A (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pLV CDHblast Rev(V16D)-EGFP-V5

Plasmid

#175165

Purpose

Lentiviral expression of Rev(V16D)-EGFP-V5

Depositor

Larry Gerace

Article

Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

Insert

rev

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V16D

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-181b-Decoy.hPGK.GFP.WPRE

Plasmid

#46595

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-181b Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.GFP.miR-639.sensor.PGK.dNGFR.WPRE

Plasmid

#85923

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-639 Sensor (MIR639 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBEG R3-L4 #BGV194

Plasmid

#48954

Purpose

For inserting selection gene between AttR3 and AttL4 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway cloning vector

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pC0.062

Plasmid

#119651

Purpose

Level 0 part. Terminator for luxICDABEG operon from Vibrio fischeri.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Bba_B0011

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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AL13Rb-HsfA2

Plasmid

#161012

Purpose

PP7-tagged reporter driven by the Arabidopsis HsfA2 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC1 (1-630)

Plasmid

#22974

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-630) (CRTC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-630 rather than 1-634.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCAGGS/Daxx myc S502A/669A

Plasmid

#19283

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
XE28 XBC 40

Plasmid

#16842

Depositor

Randall Moon

Article

Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

Insert

beta-catenin-myc (ctnnb1.L Frog)

Use

Xenopus expression

Tags

6x myc

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
pC0.017

Plasmid

#119621

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

dCas9

Use

Synthetic Biology

Mutation

bp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1A2QS

Plasmid

#138268

Purpose

MINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activity

Depositor

Xiao Wang

Article

Wu et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Inserts

AraC-TetR operon
LuxR-LacI operon
mCherry
GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Para/lac and Plux/tet

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pES002 (pTet-qacR wt)

Plasmid

#69031

Purpose

wild-type qacR downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Tags

His6 tag

Expression

Bacterial

Promoter

pTet

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMK110

Plasmid

#99535

Purpose

contains bgl operon sequence for E. coli in vitro transcription template

Depositor

Robert Landick

Article

Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife

Insert

E. coli bgl antisense region containing bglG

Tags

None

Promoter

lambda Pr promoter used for in vitro transcriptio…

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pJH2141

Plasmid

#179248

Purpose

Punc-4::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-9 (unc-9 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Punc-4

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2037

Plasmid

#179220

Purpose

Psra-11::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-7 (unc-7 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Psra-11

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEntra1a-mTurquoise2-3xrGBD

Plasmid

#176137

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

rhotekin (Rtkn Mouse)

Use

Entry vector

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 CC3D

Plasmid

#175776

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC3D

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Mutation

CC3D

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 CC2D

Plasmid

#175775

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC2D

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Mutation

CC2D

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 CC1D

Plasmid

#175774

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC1D

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Mutation

CC1D

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCA0.001

Plasmid

#119557

Purpose

Level 0 acceptor vector for promoters truncated to transcriptional start site with lacZ compatible with blue/white screening

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/intron/FlagCter

Plasmid

#113548

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

Flag

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-FKBP12-C1-DH

Plasmid

#67901

Purpose

Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12-DH

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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ShaCas9

Plasmid

#163794

Purpose

Expresses ShaCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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PI(LM)-Rac1(WT)dc1.FLARE

Plasmid

#87590

Purpose

PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDG279

Plasmid

#85219

Purpose

mammalian expression of ubiquitin-K48R-EGFP fusion

Depositor

Douglas Gray

Article

Tsirigotis et al J Biol Chem. 2001 Dec 7;276(49):46073-8.

Insert

ubiquitin C (UBC Human)

Tags

6xHIS and EGFP

Expression

Mammalian

Mutation

K48R

Promoter

human ubiquitin C

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
pRS316-RGR-GFP-mHH

Plasmid

#51057

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pPIC-mouseKir3.2-mC-S

Plasmid

#140745

Purpose

Expression of mouse Kir3.2 fused to mCherry and Streptag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

Insert

Kir3.2 (Kcnj6 Mouse)

Tags

mCherry with Streptag II

Expression

Yeast

Promoter

AOX1

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-nirButterfly

Plasmid

#136590

Purpose

Expression of a genetically-encoded voltage indicator nirButterfly

Depositor

Vladislav Verkhusha

Article

Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

Insert

nirButterfly

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Hoxc4

Plasmid

#45550

Depositor

Jeremy Dasen

Article

Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

Insert

Hoxc4 (Hoxc4 Mouse)

Promoter

CMV / b-actin

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
pBluescript-mHMGB4

Plasmid

#31620

Depositor

Yasuhiko Kawakami, Martin Lotz

Article

Itou et al Dev Dyn. 2011 May . 240(5):1151-62.

Insert

HMGB4 (Hmgb4 Mouse)

Expression

Bacterial

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA-Dest53-SHP-1 S591D

Plasmid

#21803

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 591 to Aspartic acid

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-Chronos(M140T)-mRuby2-ST

Plasmid

#109129

Purpose

Modified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

Chronos(M140T)-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFS217-USP44_MSCV-TAP

Plasmid

#43896

Purpose

Retroviral expression of USP44 in mammalian cells

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Retroviral

Tags

FLAG, HA, and HIS

Expression

Mammalian

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only
p-mCherry-N1-slowChloC

Plasmid

#85462

Purpose

Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

Depositor

Peter Hegemann

Article

Wietek et al Science. 2014 Mar 27.

Insert

slowChloC

Tags

mCherry

Expression

Mammalian

Mutation

E90R, T159C, D156N

Promoter

CMV (+enhancer)

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Tip alpha (21-192)

Plasmid

#23157

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-20

Available Since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-Dest53-SHP-1 S591A

Plasmid

#21802

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 591 to Alanine

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
pGBT9-chNfasc (+RSLE)

Plasmid

#89418

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

NFASC (NFASC Chicken)

Tags

GAL4 DBD

Expression

Yeast

Promoter

ADH1

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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mNG-mCh

Plasmid

#124215

Purpose

Cytoplasmic mNeongreen mCherry tandem, ~19 % energy transfer pNM002 - pSAV057-mNG-mCh

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

Insert

mNG-mCh

Expression

Bacterial

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 9641 - 9680 of 50988 results

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