We narrowed to 10,684 results for: plasmids 101
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Plasmid#69259Purposephlh-8::tagBFP2DepositorInsertTagBFP2
UseCre/LoxExpressionWormMutationcodon-optimzed index 1.0, TagBFP2 contains 2 sing…Promoterhlh-8Available SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-UGCG-tdTomato
Plasmid#225879PurposePlasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertionDepositorInserttdTomato
UseCRISPRExpressionMammalianAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPath-pTac-integrase
Plasmid#199999PurposeEntry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.DepositorInsertPathways
ExpressionBacterialAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_ALPPL2 synNotch
Plasmid#188376PurposeLentiviral vector - constitutive expression of an anti-ALPPL2 synNotch with a Gal4VP64 transcriptional factorDepositorInsertPGK_antiALPPL2_synNotch_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/intron/GFP
Plasmid#113547PurposeMammalian expression plasmid that can be used with the Flp-In T-REx system.DepositorInsertGFP
TagsGFPExpressionMammalianAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG:: ChR2HA-2a-hM4D
Plasmid#52520PurposeCo-expresses HA tagged channelrhodopsin and hM4D (Gi)DepositorInsertChR2-2a-hM4D
Tags2xHA on ChR2ExpressionMammalianPromoterCAGAvailable SinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
BSP188
Plasmid#110917PurposeFor cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator clonedDepositorInsertunc54 Terminator
UseSynthetic Biology; Yeast homologous recombination…ExpressionWormAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1-GeneTRAP
Plasmid#218184PurposeTo KO and genetrap mouse Hevin simultaneouslyDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1-N-HATag
Plasmid#218185PurposeTo tag Hevin with HA at the N-terminalDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1 KO
Plasmid#218182PurposeTo KO Hevin in astrocytesDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSR40
Plasmid#69257Purposeplin-31::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterlin-31Available SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB036d
Plasmid#99216PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PproAAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB036a
Plasmid#99215PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK and PproDAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC15-P1B3
Plasmid#202856PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC15DepositorInsertEGFP/BC15
ExpressionMammalianPromoterTet Response Element (TRE)Available SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC11-P1A11
Plasmid#202852PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC11DepositorInsertEGFP/BC11
ExpressionMammalianPromoterTet Response Element (TRE)Available SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRPa-iSL(MCS1+2)
Plasmid#69244PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiPromoterRRNA promoterAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR33
Plasmid#69254Purposephsp16.2::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterhsp16.2Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Let7-SunTag
Plasmid#196928PurposeLet7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7R-SunTag
Plasmid#196929PurposeReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC82-P1G10
Plasmid#202923PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82DepositorInsertEGFP/BC82
ExpressionMammalianPromoterTet Response Element (TRE)Available SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only