We narrowed to 11,195 results for: AGA

1. spCas9-NLS-MAV

   Plasmid

   #124212

   Purpose

   SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

   Depositor

   Uri David Akavia

   Article

   Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   His6, MBP, TEV Cleavage site, and two copies of S…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA

   Plasmid

   #108748

   Purpose

   Expresses aa28 to C-terminus of Flp recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment aa28 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgRNA-Cre-GpNLuc

   Plasmid

   #208382

   Purpose

   Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Type

   Empty backbone

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCK036 desmaMCS:minCerulean,cryaa:Venus

   Plasmid

   #195981

   Purpose

   Expression construct

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, cryaa Venus (desma Zebrafish)
   

   Use

   Other

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAPM-miR30-DDX58-ts3

   Plasmid

   #174243

   Purpose

   DDX58 knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   DDX58 shRNA (RIGI Human)
   

   Use

   Lentiviral and RNAi

   Promoter

   SFFV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG/ATP11C(E184Q)-HA

   Plasmid

   #209251

   Purpose

   Mammalian expression of ATP11C mutant E184Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP11C (ATP11C Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E184Q

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG/ATP8A1(E191Q)-HA

   Plasmid

   #209240

   Purpose

   Mammalian expression of ATP8A1 mutant E191Q

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

   Insert

   ATP8A1 (ATP8A1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E191Q

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTargetF-FRT

   Plasmid

   #113637

   Purpose

   Derivative of pTargetF with gRNA targeting FRT site

   Depositor

   Olivier Lichtarge

   Article

   Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

   Insert

   sgRNA-FRT
   

   Use

   CRISPR

   Promoter

   pJ23119

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28aAviHisCD72cCTLD

   Plasmid

   #129066

   Purpose

   generation of biotinylated mouse CD72c CTLD protein using bacteria

   Depositor

   Takeshi Tsubata

   Article

   Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

   Insert

   CD72c (Cd72 Mouse)
   

   Expression

   Bacterial

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. p168-759

    Plasmid

    #62619

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. L2_lacZgRNA-Cas9-CsA

    Plasmid

    #136138

    Purpose

    Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAT-YFP-TetR

    Plasmid

    #59018

    Purpose

    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii

    Depositor

    David Sibley

    Article

    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

    Insert

    YFP-TetR
    

    Use

    Toxoplasma expression

    Promoter

    alpha-tubulin

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

    Plasmid

    #185676

    Purpose

    SpCas9 and gRNA targeting the C-terminus of HsIRE1

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 gRNA (ERN1 Human)
    

    Use

    CRISPR

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

    Plasmid

    #170177

    Purpose

    Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2s-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCK069 desmaMCS:minprom_mApple,exorh:EGFP

    Plasmid

    #195982

    Purpose

    Expression construct

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
    

    Use

    Other

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. plentiCRISPv2_AAVS1

    Plasmid

    #184487

    Purpose

    Lentivirus all in one CRISPR vector targeting human AAVS1 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    AAVS1 (AAVS1 Human)
    

    Use

    Lentiviral

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-lincRNA-RoR

    Plasmid

    #45763

    Depositor

    George Daley

    Article

    Loewer et al Nat Genet. 2010 Dec;42(12):1113-7. Epub

    Insert

    lincRNA-RoR cDNA (LINC-ROR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-TRIM66

    Plasmid

    #65400

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    TRIM66 (TRIM66 Human)
    

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

    Plasmid

    #172984

    Purpose

    CRISPRi for TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    sgTAOK2i (TAOK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-SP140

    Plasmid

    #65394

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SP140 (SP140 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only