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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,995 results for: RAN-1

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Showing: 4841 - 4860 of 19995 results

  1. pVG122

    Plasmid
    #211765
    Purpose
    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
    Depositor
    Sahand Jamal Rahi
    Article
    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:
    Insert
    strongLOV
    Use
    For single-copy integration (if cut with pmei)
    Tags
    SV40 NLS and VP16
    Expression
    Yeast
    Mutation
    compared to EL222, strongLOV contains Glu84Asp mu…
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211765-plasmid-map-sequence-id-417945

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  2. DPAGT1 (DPAGT1A-c005)

    Plasmid
    #98228
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DPAGT1A (DPAGT1 Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Mutation
    pfam00953:Glycos_transf_4 98-275
    Promoter
    polyhedrin
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98228-plasmid-map-sequence-id-193399

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  6. pCR197

    Plasmid
    #240643
    Purpose
    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    Use
    Gene expression and genomic integration in fish
    Tags
    NES (from Exaiptasia diaphana MEK2), NLS (from SV…
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240643-plasmid-map-sequence-id-482910

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  11. pMA_Int

    Plasmid
    #110096
    Purpose
    E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Insert
    mycophage integrase
    Use
    Required for transformation of attb integrating v…
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110096-plasmid-map-sequence-id-211299

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  14. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  15. pGR596

    Plasmid
    #213787
    Purpose
    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    See manuscript
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213787-plasmid-map-sequence-id-424625

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  16. pAAV-hSyn-fDIO-HA-KORD-IRES-mCitrine-WPREpA

    Plasmid
    #154871
    Purpose
    Human synapsin-1 promoter; Flp-dependent KORD-IRES-mCitrine
    Depositor
    Ulrik Gether
    Insert
    KORD-IRES-mCitrine
    Use
    AAV; Flp-frt
    Tags
    HA
    Expression
    Mammalian
    Promoter
    Human Synapsin-1
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154871-plasmid-map-sequence-id-308834

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  17. pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA

    Plasmid
    #154869
    Purpose
    Human synapsin-1 promoter; Flp-dependent rM3D(Gs) DREADD-mCherry
    Depositor
    Ulrik Gether
    Insert
    rM3D(Gs)
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Human Synapsin-1
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154869-plasmid-map-sequence-id-308828

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  18. pAAV-hPhyB621(Y276H)-P2A-m1V

    Plasmid
    #197377
    Purpose
    The AAV vector for PhyB621(Y276H) and m1V
    Depositor
    Michiyuki Matsuda
    Insert
    Phytochrome B Y276H(1-621 aa)
    Use
    AAV
    Tags
    P2A-m1V
    Mutation
    Human codon optimized PhyB (1-621 aa), Y276H muta…
    Promoter
    CMV
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197377-plasmid-map-sequence-id-389799

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Showing: 4841 - 4860 of 19995 results