We narrowed to 14,321 results for: cas9

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dCas9 fused to nanobody (anti-beta catenin)

Plasmid

#186420

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

Depositor

Stephen Elledge

Publication

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-nanobody fusion (anti-beta catenin)

Use

CRISPR

Tags

6His

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

Plasmid

#223159

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Publication

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

Plasmid

#135978

Purpose

gRNA against the right loxP site of the Ai9 allele

Depositor

William Lagor

Insert

Ai9 gRNA2

Use

AAV, CRISPR, and Mouse Targeting

Tags

HA

Promoter

CB

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

Plasmid

#117405

Purpose

SpyCas9 sgRNA 1 targeting TLR2.0

Depositor

Erik Sontheimer

Publication

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SpyCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

Plasmid

#102891

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Publication

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-P300-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

Plasmid

#71214

Purpose

dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Publication

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Tags

dSpCas9-BFP-DNMT3B(E697A)

Expression

Mammalian

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRCA422 - pHR-UCOE-SFFV-VPH-dcas9-p2a-mCherry

Plasmid

#238165

Purpose

Express VPH-dCas9-P2A-mCherry in mammalian cells. Lentiviral

Depositor

Thomas Norman

Publication

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

VPH-dCas9-P2A-mCherry

Use

CRISPR and Lentiviral

Tags

mCherry

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_UCOE-EF1a-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

Plasmid

#188769

Purpose

dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Promoter

EF1a

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Julianna (split-vector) (Lentiviral Prep)

Viral prep

#247028-LV

Purpose

Ready-to-use Lentiviral Prep particles produced from Julianna (split-vector) (#247028). In addition to the viral particles, you will also receive purified Julianna (split-vector) plasmid DNA.

Depositor

John Doench, David Root

Publication

Drepanos et al Cell Genom. 2026 May 13;6(5):101190. doi

Available since

May 11, 2026

Availability

Academic Institutions and Nonprofits only
pSpCas9 UGT1 (pWZ594)

Plasmid

#254037

Purpose

Express SpCas9 and the UGT1 gRNA

Depositor

Jef Boeke

Publication

Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

gRNA_UGT1

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pSpCas9 LP400~3p (pKBA314)

Plasmid

#254039

Purpose

Express SpCas9 and the LP400~3p gRNA

Depositor

Jef Boeke

Publication

Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

gRNA_LP400~3p

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pSpCas9 UNS1 (pKBA244)

Plasmid

#254040

Purpose

Express SpCas9 and the UNS1 gRNA

Depositor

Jef Boeke

Publication

Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

gRNA_UNS1

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pSpCas9 UNS2 (pKBA235)

Plasmid

#254041

Purpose

Express SpCas9 and the UNS2 gRNA

Depositor

Jef Boeke

Publication

Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

gRNA_UNS2

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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ROS1-dCas9 (D10)

Plasmid

#239487

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Publication

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-ROS1-XTEN80-NLS-dCas9-2xNLS-P2A-BFP

Use

CRISPR

Expression

Plant

Available since

Feb. 17, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9

Plasmid

#237460

Purpose

Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

3xFlag_ABE8e_SpCas9(D10A)_6xNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A

Promoter

chicken beta-actin promoter & U6

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX462-ABE8e-nSaCas9

Plasmid

#237462

Purpose

Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSaCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

3xFlag_ABE8e_SaCas9 -6xNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A

Promoter

chicken beta-actin promoter & U6

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9 (M14)

Plasmid

#239482

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Publication

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-P2A-BFP

Use

CRISPR

Expression

Plant

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPD0028_pIVTRup(BsmBI)_dCas9

Plasmid

#242536

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Publication

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

dCas9

Use

CRISPR

Mutation

D10A

Available since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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Cp PS Intein dCas9-VPR

Plasmid

#242031

Purpose

Blue-light-controlled release of dCas9-VPR from the cytoplasm to the nucleus.

Depositor

Vladislav Verkhusha

Publication

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Cp PS Intein dCas9-VPR

Expression

Mammalian

Promoter

CMV

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-NUP98a_IDR-EGFP

Plasmid

#232766

Purpose

Expresses dCas9-NUP98a_IDR-EGFP

Depositor

Isaac Hilton

Publication

Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

Insert

dCas9-NUP98a_IDR-EGFP (NUP98 Human)

Use

Lentiviral

Promoter

EF1a

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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