We narrowed to 59,537 results for: cloning

1. pFH35

   Plasmid

   #126009

   Purpose

   Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PTPN11 in pENTR

   Plasmid

   #216604

   Purpose

   gateway cloning donor vector containing PTPN11

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   PTPN11 (PTPN11 Human)
   

   Use

   Gateway entry plasmid

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEntr_PE2

   Plasmid

   #149548

   Purpose

   Gateway entry clone with PE2 enzyme (with stop codon)

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   PE2
   

   Use

   CRISPR; Gateway shuttle vector

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH41

   Plasmid

   #125880

   Purpose

   Level0 Parsley PcUBI4-2 Pol II promoter

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   Level0 Parsley PcUbi4-2 Pol II promoter
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK-N. tabacum trnI/trnA

   Plasmid

   #123579

   Purpose

   MoChlo kit

   Depositor

   Scott Lenaghan, C. Neal Stewart

   Article

   Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

   Insert

   N. tabacum trnI/trnA integration site
   

   Use

   Chloroplast transformation vector

   Expression

   Plant

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO HIS

   Plasmid

   #19446

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HIS
   

   Expression

   Mammalian

   Mutation

   HIS tag inserted to create new vector

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pECNUS_MP02

   Plasmid

   #194351

   Purpose

   CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

   Depositor

   Eunyoung Chae

   Article

   Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

   Insert

   pcoCas9
   

   Expression

   Plant

   Promoter

   RPS5A

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD364

   Plasmid

   #91831

   Purpose

   Lox511I-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Lox511I-SEC-Lox511I
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. EHMT1 (2IGQ)

   Plasmid

   #25504

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EHMT1 (EHMT1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   C-terminal domain, residues 951-1235

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

10. p663-UBC-miniTurbo-V5-TP53RK_IDG-K

    Plasmid

    #170589

    Purpose

    Gateway destination clone of TP53RK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-TP53RK (TP53RK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDD382

    Plasmid

    #91830

    Purpose

    Lox2272-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox2272-SEC-Lox2272
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOGG026

    Plasmid

    #113992

    Purpose

    Level 1 golden gate cloning vector for low copy number stable environmental gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. p{Mhc-F4-678-DsRed::GW,attB}

    Plasmid

    #182171

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Expression

    Insect

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. BASIC_3_Kan

    Plasmid

    #68137

    Purpose

    storage vector for BASIC part: Kan

    Depositor

    Geoffrey Baldwin

    Article

    Storch et al ACS Synth Biol. 2015 Apr 16.

    Insert

    Kanamycin
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. BASIC_4_Cm

    Plasmid

    #68138

    Purpose

    storage vector for BASIC part: Cm

    Depositor

    Geoffrey Baldwin

    Article

    Storch et al ACS Synth Biol. 2015 Apr 16.

    Insert

    Chloramphenicol
    

    Expression

    Bacterial

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOGG024

    Plasmid

    #113991

    Purpose

    Level 1 golden gate cloning vector for medium copy number free-living expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAMP-CY1_NF1 T2 (+minintr)

    Plasmid

    #119205

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 2 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFOSGE_COF4C01

    Plasmid

    #71009

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Cand1 (CG5366 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAK-EL-03

    Plasmid

    #125762

    Purpose

    Endlinker for tRNA-gRNA system when using only three guides

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Endlinker
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFH66

    Plasmid

    #131765

    Purpose

    Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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