We narrowed to 62,258 results for: cloning

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Human J-chain

Plasmid

#158215

Purpose

Express the human J-chain

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

JCHAIN (JCHAIN Human)

Tags

8×his

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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RKS-Fzd7/8 subtype NGS Wnt

Plasmid

#159628

Purpose

Express Fzd7/8 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1amCherry-2A-OsTIR1-W

Plasmid

#159296

Purpose

Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

OsTIR

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a promoter

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-His-SCov2-nsp7

Plasmid

#154757

Purpose

Expression of SARS-CoV-2 nsp7

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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iOn-CAG∞RFP

Plasmid

#154014

Purpose

Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlpha15-RLuc8

Plasmid

#140984

Purpose

Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlpha15-RLuc8 (GNA15 Human)

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0

Plasmid

#153288

Purpose

Express the adenosine sensor GRAB_Ado1.0 in astrocytes

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

GfaABC1D

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-AdEvolve-DEST

Plasmid

#131692

Purpose

A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Type

Empty backbone

Use

Adenoviral

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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PL-5LTR-RGR(DMD#1)-AmCyan-A

Plasmid

#138482

Purpose

Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Ribozyme-flanked gRNA and AmCyan

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFRm43GW

Plasmid

#133748

Purpose

Three-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thaliana

Depositor

Niko Geldner

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFG7m34GW

Plasmid

#133747

Purpose

Three-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana

Depositor

Niko Geldner

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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DR5rev::erRFP

Plasmid

#61011

Purpose

Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

Depositor

Alexis Maizel

Article

Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

Insert

DR5rev::erRFP:35S_term

Expression

Plant

Promoter

DR5rev::erRFP:35Sterm

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:GtACR2-tdTomato

Plasmid

#124236

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR2-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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mARG1 cassette 3

Plasmid

#134345

Purpose

Mammalian acoustic reporter gene 1 cassette 3 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 3

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX305_FKBP12F36V-SHOC2

Plasmid

#134522

Purpose

Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

Depositor

Andrew Aguirre

Article

Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Tags

FKBP12F36V-2xHA

Expression

Mammalian

Mutation

Silent mutations made at wobble base position at …

Promoter

hPGK

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET23b-2E2-HA-mEGFP-6xHis

Plasmid

#129594

Purpose

The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR_L1-YFP-Turbo-NES-L2

Plasmid

#127349

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, NES, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCpf1b-sp

Plasmid

#122188

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTOX12

Plasmid

#127457

Purpose

Encodes Tse2 toxin. Gent-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

tse2 toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oHex

Plasmid

#124670

Purpose

Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHex

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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