We narrowed to 6,993 results for: CAD

1. pCas3cRh

   Plasmid

   #133773

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Joseph Bondy-Denomy

   Article

   Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   Cas7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. hOT-GFP

   Plasmid

   #67848

   Purpose

   mammalian expression of human oxytocin receptor with GFP tag

   Depositor

   Christian Gruber

   Article

   Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

   Insert

   oxytocin receptor (OXTR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

3. iCAP-BI-28-KanR

   Plasmid

   #203532

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI28 Cap
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a (+)-hTFIIB

   Plasmid

   #171084

   Purpose

   Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   TF2B (GTF2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX5X.1 PINK1 WT

   Plasmid

   #13321

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 kinase domain (PINK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Amino acids 112-496

   Promoter

   Available since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-Axin1

   Plasmid

   #109370

   Purpose

   Transient over-expression of human Axin1

   Depositor

   Mariann Bienz

   Article

   Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

   Insert

   Axin1 (AXIN1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LRRK2-WT

   Plasmid

   #17609

   Depositor

   Ted Dawson

   Article

   West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-NFE2L2-Puro

   Plasmid

   #181919

   Purpose

   Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

   Depositor

   Scott Dixon

   Article

   Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

   Insert

   NFE2L2 (NFE2L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiGuide Puro-P2A-EGFP

   Plasmid

   #137729

   Purpose

   For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-ArgiNLS-EGFP

    Plasmid

    #220594

    Purpose

    Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    EGFP
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLViP-LifeAct-iRFP670

    Plasmid

    #229699

    Purpose

    Lentiviral expression of LifeAct-iRFP670 in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    Synthetic construct LifeAct-mTurquoise gene
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry-Synaptojanin-1-145

    Plasmid

    #223563

    Purpose

    Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

    Depositor

    Pietro De Camilli

    Article

    Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Use

    Tags

    His and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

    Plasmid

    #184464

    Purpose

    Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMax_ScaI_BFP

    Plasmid

    #177824

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' inserted ScaI RE cut site

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWPI-N3ICD

    Plasmid

    #185524

    Purpose

    Constitutive overexpression of human NOTCH3 intracellular domain

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH3 intracellular domain (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-ArgiNLS-mGreenLantern

    Plasmid

    #220596

    Purpose

    Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1036 pcDNA3 Myr HA Akt1

    Plasmid

    #9008

    Purpose

    Mammalian expression of human Akt1 with myr, HA tags

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL046

    Plasmid

    #198807

    Purpose

    In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe puro HA PIK3CA H1047R

    Plasmid

    #12524

    Purpose

    Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

    Depositor

    Jean Zhao

    Article

    Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H1047R. Tumor-derived allele.

    Promoter

    Available since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEmerald(luminal)-canxTM

    Plasmid

    #186961

    Purpose

    Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) with ER signal pe…

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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