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pRSET iGluSnFR3 v857

Plasmid

#175186

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

iGluSnFR3 v857

Use

Synthetic Biology

Tags

His tag, T7 leader, Xpress epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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VAMP3-mCherry

Plasmid

#92423

Purpose

Expression of VAMP3 C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBIS-GALkFLP(URA)

Plasmid

#49460

Purpose

overexpression of Flp step-arrest mutant in yeast, URA selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pγsat

Plasmid

#39238

Depositor

Niall Dillon

Article

Lundgren et al Cell. 2000 Nov 22;103(5):733-43.

Insert

8 copies of the 234bp mouse γ-satellite repeat

Use

Cloning vector

Available Since

Jan. 16, 2013

Availability

Academic Institutions and Nonprofits only
MSCVhygro-F-G9adSET

Plasmid

#41722

Purpose

Retroviral expression of human G9a mutant in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9adset (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pActP-GCaMP6m

Plasmid

#64714

Purpose

visualizing calcium in cultured Drosophila S2 cells

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

GCaMP6m

Expression

Insect

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-AKT

Plasmid

#20281

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

AKT (AKT1 Human)

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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YPet-pBAD

Plasmid

#54860

Purpose

Localization: Protein Expression Vector , Excitation: 513, Emission: 527

Depositor

Patrick Daugherty, Michael Davidson

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Type

Empty backbone

Tags

6xHis and YPet

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Sctrans-PP

Pooled Library

#153101

Purpose

CRISPR library targets all non-essential and conditionally essential transporter genes in S. cerevisiae

Depositor

Irina Borodina

Article

Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(

Expression

Yeast

Species

Saccharomyces cerevisiae

Use

CRISPR

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV1)

Viral Prep

#125712-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Promoter

short version of αCamKinase II promoter (0.4kb)

Tags

mScarlet

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only
pGAL4-DBD-MCS

Plasmid

#145245

Purpose

Backbone vector expressing GAL4 DNA-binding domain followed by MCS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon oG

Plasmid

#131778

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

rabies glycoprotein - oG

Use

AAV; Intrsect

Tags

none

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGRG36-Kn-PA1-GFP

Plasmid

#79088

Purpose

pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

Depositor

Frank Rosenzweig

Article

Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

Insert

GFP

Use

Bacterial chromosome integration and expression o…

Expression

Bacterial

Promoter

PA1

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-VSVG

Plasmid

#80606

Purpose

Mammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the protein

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

VSV-G

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA1 Flag ATXN1[2Q]

Plasmid

#33235

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Tags

Flag

Expression

Mammalian

Mutation

insert contains 2Q (2 glutamines) and there isno …

Promoter

CMV

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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p043 mTcf-4 B

Plasmid

#11031

Depositor

Ramesh Shivdasani

Article

Lee et al J Biol Chem. 1999 Jan 15. 274(3):1566-72

Insert

Tcf-4 (Tcf7l2 Mouse)

Use

Xenopus, avian, and zebrafish expression

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pAd:TRE-gateway

Plasmid

#30217

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Type

Empty backbone

Use

Adenoviral

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
YPet-C1

Plasmid

#54648

Purpose

Localization: C1 Cloning Vector, Excitation: 513, Emission: 527

Depositor

Patrick Daugherty, Michael Davidson

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Type

Empty backbone

Tags

YPet

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCRISPRi-v2, Mitochondria, Trafficking, and Motility (m4), top 5 sgRNAs/gene

Pooled Library

#83992

Purpose

Mouse CRISPRi Pooled Library targeting Mitochondria, Trafficking, and Motility genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
pT1UciMP

Plasmid

#62215

Purpose

UAS transgenesis vector

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Promoter

UAS-E1b

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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