We narrowed to 32,895 results for: Eng
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Plasmid#178569PurposeConstitutive expression of a gRNA-tRNA array for macsGESTALT editingDepositorInsert5 gRNA-tRNA array with U6 promoter
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
TagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYC-N
Plasmid#207113PurposeHDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locusDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB
Plasmid#79050PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
TagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-FnCas12a-TEV
Plasmid#174658PurposeFnCas12a expression plasmidDepositorInsertFnCas12a
UseCRISPRExpressionBacterialAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-M2
Plasmid#135584PurposeHigh eukaryotic dual protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorExpressionInsectPromoterpolyhedrin of AcMNPVAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorInsertcAMPFIRE-L (cAMP sensor)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70
Plasmid#15215DepositorAvailable SinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI
Plasmid#246754PurposeRNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.DepositorInsertCIRC-V1-Ana-CVB3 IRES-EGFP-BbsI
UseSynthetic BiologyAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCambia3301-AtU6-MAR1
Plasmid#210757PurposeMutagenesis of the Arabidopsis MAR1 gene for efficient screening.DepositorInsertAtU6-26 pro::MAR1 sgRNA
UseCRISPRExpressionPlantPromoterAtU6-26Available SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAzurite-16xRhoBAST
Plasmid#196347PurposeExpression of mAzurite mRNA with 16xRhoBAST in 3'UTRDepositorInsertmAzurite-16xRhoBAST
ExpressionMammalianPromoterCMVAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI
Plasmid#246755PurposeRNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BsaI.DepositorInsertCIRC-V1-Ana-CVB3 IRES-EGFP-BsaI
UseSynthetic BiologyAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYC-C
Plasmid#207114PurposeHDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locusDepositorTypeEmpty backboneUseCRISPRAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
double cut NH-donor
Plasmid#83576PurposeeGFP reporter donor containing double SA cutting sites for NHEJ knock-inDepositorInserteGFP
UseNhej donorAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
T2A 3xNLS GFP
Plasmid#227721PurposePlasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertT2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
UseCloning vectorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFW2100
Plasmid#224212PurposeBacteroides genomic editing tool; IPTG inducible-Fncpf1DepositorInsertFncpf1
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT57
Plasmid#223429PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
T2A GFP H2B
Plasmid#227722PurposePlasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp ResistantDepositorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
hCas9_D10A
Plasmid#41816PurposeExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineeringDepositorInsertCas9_D10A
UseCRISPRExpressionMammalianMutationhuman codon-optimized, D10A nickasePromoterCMVAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only