We narrowed to 32,602 results for: Eng

1. pILGFP4K

   Plasmid

   #104506

   Purpose

   Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   SpGAL1 promoter-yEGFP
   

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFP4O

   Plasmid

   #104510

   Purpose

   Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   SpdGAL2 promoter-yEGFP
   

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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3. pILGFP4P

   Plasmid

   #104511

   Purpose

   Used to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter gene

   Depositor

   Claudia Vickers

   Article

   Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

   Insert

   SptGAL2 promoter-yEGFP
   

   Expression

   Yeast

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-jGCaMP7b-XC

   Plasmid

   #182221

   Purpose

   Improved genetic encoded calcium sensor based on GCaMP7b.

   Depositor

   Xiaodong Liu

   Article

   Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

   Insert

   jGCaMP7b-XC
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUltra-U6-gRNAs1-5

   Plasmid

   #178569

   Purpose

   Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

   Depositor

   Christopher Lengner

   Article

   Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

   Insert

   5 gRNA-tRNA array with U6 promoter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP_mNG2(11)_Clathrin light chain

   Plasmid

   #82608

   Purpose

   Expresses mNG2(11) tagged clathrin light chain in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   mNG2(11)_Clathrin light chain
   

   Expression

   Mammalian

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. mAzurite-16xRhoBAST

   Plasmid

   #196347

   Purpose

   Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

   Depositor

   Murat Sunbul

   Article

   Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

   Insert

   mAzurite-16xRhoBAST
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYC-C

   Plasmid

   #207114

   Purpose

   HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

   Depositor

   Yifan Cheng

   Article

   Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYC-N

   Plasmid

   #207113

   Purpose

   HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

   Depositor

   Yifan Cheng

   Article

   Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI

    Plasmid

    #246755

    Purpose

    RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BsaI.

    Depositor

    Wensheng Wei

    Article

    Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

    Insert

    CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI
    

    Use

    Synthetic Biology

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI

    Plasmid

    #246757

    Purpose

    RNA circularization with CIRC using Azo intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

    Depositor

    Wensheng Wei

    Article

    Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

    Insert

    CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQAT51

    Plasmid

    #223423

    Purpose

    T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQAT57

    Plasmid

    #223429

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTK-EdnrbE2-mCherry

    Plasmid

    #127772

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer E2 driving mCherry activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE2
    

    Use

    Enhancer reporter construct

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI

    Plasmid

    #246754

    Purpose

    RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

    Depositor

    Wensheng Wei

    Article

    Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

    Insert

    CIRC-V1-Ana-CVB3 IRES-EGFP-BbsI
    

    Use

    Synthetic Biology

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. ND6-L1397-N_UGI-NES_right

    Plasmid

    #187846

    Purpose

    expresses the right-side half ND6-UGI-NES in mammalian cells

    Depositor

    Chengqi Yi

    Article

    Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

    Insert

    COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ND6-L1397-N_UGI-NES_left

    Plasmid

    #187845

    Purpose

    expresses the left-side half ND6-UGI-NES in mammalian cells

    Depositor

    Chengqi Yi

    Article

    Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

    Insert

    SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTK-EdnrbE1-Citrine

    Plasmid

    #127773

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE1
    

    Use

    Enhancer reporter construct

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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