We narrowed to 24,897 results for: Nes;

1. ML25

   Bacterial Strain

   #61916

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG asnA asnB genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

2. ML26

   Bacterial Strain

   #61917

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

3. ML36

   Bacterial Strain

   #61919

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG metA genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

4. ML31

   Bacterial Strain

   #61918

   Purpose

   C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

5. C-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58694

   Purpose

   Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
   

   Use

   AAV and CRISPR

   Tags

   GyrA Csplit Intein and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. N-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58693

   Purpose

   Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
   

   Use

   AAV and CRISPR

   Tags

   3xFlag, GyrA Nsplit Intein, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNK3071

   Plasmid

   #219755

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNK497

   Plasmid

   #219754

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-CBP FL

   Plasmid

   #179550

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-Cas9-2A-BFP

    Plasmid

    #164662

    Purpose

    Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Inserts

    Cas9
    BFP
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSSV_LTR

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-PHFF-sh-hBVRA

    Plasmid

    #100285

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-GCN5 FL

    Plasmid

    #179552

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. LMPd Amt OXCT1

    Plasmid

    #209407

    Purpose

    Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Luda et al Immunity. 2023 Sep 12;56(9):2021-2035.e8

    Insert

    Oxct1 shRNA (Oxct1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. ABL1_HUMAN_D0

    Plasmid

    #79727

    Purpose

    This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    ABL1 (ABL1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. G protein alpha-s-YFP

    Plasmid

    #55781

    Purpose

    This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    alpha-s-EE YFP (Gnas Rat)
    

    Use

    Tags

    EYFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    Met was substituted for Gln69 in EYFP (Clontech).…

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2K7bsdUBI-mCherry-STIM1

    Plasmid

    #114178

    Purpose

    Lentiviral vector for expression of mCherry-STIM1

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry (inserted after signal peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLIK Zeo p53DD

    Plasmid

    #136534

    Purpose

    Lentiviral expression vector for an inducible p53DD

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    p53DD (Trp53 Mouse)
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    Expression

    Mammalian

    Mutation

    This construct was created from the pBabe p53DD (…

    Promoter

    Available since

    Feb. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dyn1(K44A)-mRFP

    Plasmid

    #55795

    Purpose

    Dominant negative Dynamin 1 mutant tagged with mRFP

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Dynamin-1(K44A)-mRFP (Dnm1 Discosoma sp., Rat)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Dynamin-1(K44A)-mRFP was produced from Dynamin-1(…

    Promoter

    CMV

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYX233-b2-DHFR

    Plasmid

    #163759

    Purpose

    Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    b2-DHFR (Dhfr Budding Yeast, Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

    Promoter

    GAL1

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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