We narrowed to 13,665 results for: crispr cas9

1. pMLS236

   Plasmid

   #73684

   Purpose

   SapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMLS235

   Plasmid

   #73683

   Purpose

   SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiGuide-Puro.3xBsmBI

   Plasmid

   #196709

   Purpose

   Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF525-AcrIIA13b_gb76_GF31-2-hom_lenti

   Plasmid

   #138310

   Purpose

   Lenti expression of AcrIIA13b

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA13b
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF525-AcrIIA15_gb79_Cand27_lenti

   Plasmid

   #138313

   Purpose

   Lenti expression of AcrIIA15

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA15
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCF525-AcrIIA14_gb77_Cand9_lenti

   Plasmid

   #138311

   Purpose

   Lenti expression of AcrIIA14

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA14
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAEF6

   Plasmid

   #136303

   Purpose

   pAEF5 plasmid with two gRNA inserted

   Depositor

   Gilles Fischer

   Article

   Agier et al Methods Mol Biol. 2021;2196:181-198. doi

   Insert

   double cassette coding for gRNA
   

   Use

   CRISPR; Template for pcr to insert double grna co…

   Available Since

   Feb. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT22

   Plasmid

   #204311

   Purpose

   For generating targeting inversions. N-terminal split GFP, expressed in eyes.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-GFPn
   

   Use

   CRISPR

   Promoter

   3XP3

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOC5 cloning template vector

   Plasmid

   #183424

   Purpose

   FlpOFF knock-in vector

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOC3 cloning template vector

    Plasmid

    #183422

    Purpose

    CreON FlpOFF knock-in vector

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKEW395_AcrIIA14_truncated

    Plasmid

    #138284

    Purpose

    Expression of truncated AcrIIA14 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    truncated AcrIIA14
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKEW398_AcrIIA15_truncated

    Plasmid

    #138286

    Purpose

    Expression of truncated AcrIIA15 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA15
    

    Use

    CRISPR

    Tags

    SFGFP

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKEW353_AcrIIA13_truncated

    Plasmid

    #138282

    Purpose

    Expression of truncated AcrIIA13 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    truncated AcrIIA13
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. NM-01

    Plasmid

    #69799

    Purpose

    Bacterial expression of Cas9 with His, MBP, and Strep tags

    Depositor

    Gerard Cagney

    Insert

    Cas9
    

    Tags

    HIS, MBP, and StrepII

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

15. C114m

    Plasmid

    #121013

    Purpose

    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    dCas9 with no bbsI or BsaI sites
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

    Plasmid

    #216421

    Purpose

    Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    3xmyc

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPbU6_hdhfr/yfcu

    Plasmid

    #216422

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBECKP-spe

    Plasmid

    #117236

    Purpose

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCBC-DT1T2

    Plasmid

    #50590

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, U626t plus, U629p, T2
    

    Use

    CRISPR; Pcr template

    Expression

    Plant

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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