Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 10,414 results for: 158

Showing: 7411 - 7440 of 10414 results

  2. AXBE

    Plasmid
    #204605
    Purpose
    Mammalian expression of adenine transversion base editor AXBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204605-plasmid-map-sequence-id-404718

    Enlarge

  6. pAS134

    Plasmid
    #211505
    Purpose
    AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    Venus
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211505-plasmid-map-sequence-id-417792

    Enlarge

  7. pAS132

    Plasmid
    #211503
    Purpose
    AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    mCherry
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211503-plasmid-map-sequence-id-417791

    Enlarge

  9. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid
    #130640
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6
    Use
    CRISPR; Transposon
    Tags
    His10-MBP-TEVsite on TniQ
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130640-plasmid-map-sequence-id-255648

    Enlarge

  13. pDT386

    Plasmid
    #174733
    Purpose
    Expresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    DT386
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Deleted amino acids 412-560 (the receptor binding…
    Promoter
    T5
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174733-plasmid-map-sequence-id-343525

    Enlarge

  21. SpCas9-NG-CGBE1

    Plasmid
    #198537
    Purpose
    Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-CGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198537-plasmid-map-sequence-id-388909

    Enlarge

  22. pSL0828 (pQCascade_crRNA-4)

    Plasmid
    #130637
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130637-plasmid-map-sequence-id-255021

    Enlarge

  26. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

    Enlarge

  27. xCas9

    Plasmid
    #138565
    Purpose
    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    xCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138565-plasmid-map-sequence-id-274797

    Enlarge

  28. BtuF

    Plasmid
    #92209
    Purpose
    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter
    Depositor
    Douglas Rees
    Article
    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99
    Insert
    BtuF (btuF )
    Use
    Tags
    6x His Tag and PelB Signal Sequence
    Expression
    Bacterial
    Mutation
    BtuF signal sequence removed and uses the PelB si…
    Promoter
    T7 Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92209-plasmid-map-sequence-id-183562

    Enlarge

  30. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid
    #213965
    Purpose
    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o
    Depositor
    Wensheng Wei
    Article
    Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213965-plasmid-map-sequence-id-422887

    Enlarge

Showing: 7411 - 7440 of 10414 results