We narrowed to 15,981 results for: LEA

1. pJL057

   Plasmid

   #177169

   Purpose

   Expresses a nuclease dead MRE11 in human cells

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   MRE11 H129N (MRE11 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   nuclease-inactivating mutation (H129N)

   Promoter

   EF1a

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY011 (pcDNA3.1-hLb2Cpf1)

   Plasmid

   #69983

   Purpose

   Expresses humanized Lb2Cpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLb2Cpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. R26-H2B-mCherry HR donor vector

   Plasmid

   #137928

   Purpose

   R0SA26 knock-in vector with homologous recombination-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-H2B-mCherry-bpA with homology arms
   

   Use

   Mouse Targeting

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPPEM

   Plasmid

   #162467

   Purpose

   Destination Expression vector for monocot plant primer editing

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Insert

   Cas9H840A
   

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine

   Promoter

   maize Ubi

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mucin MUC1-5TR-pET15b

   Plasmid

   #174694

   Purpose

   Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

   Depositor

   Jean Gariepy

   Article

   Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

   Insert

   MUC1-TR
   

   Tags

   hexahistidine (HIS) tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 Promoter

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTU424

   Plasmid

   #178983

   Purpose

   pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Insert

   TPR-CHAT
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-ChRger1-TS-EYFP

   Plasmid

   #127243

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger1-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. E3020X

   Plasmid

   #21366

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Expression

   Mammalian

   Mutation

   Changed E3020 to stop codon

   Available Since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

9. ND4.2-Left TALE-G1397-N-DddA6

   Plasmid

   #179680

   Purpose

   Expression of base editor in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (Q1310R S1330I T1380I)_1xUGI_ATP5B 3'UTR
   

   Expression

   Mammalian

   Mutation

   Q1310R, S1330I, T1380I

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. gRNA-leu-HYB

    Plasmid

    #64332

    Purpose

    leu2 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of leu2 deletion gRNA cassette
    

    Expression

    Yeast

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

    Plasmid

    #129708

    Purpose

    Express cre-dependent KASH-EGFP and scramble shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. CANE-LV envelope

    Plasmid

    #86666

    Purpose

    pCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirus

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    CANE-LV envelope
    

    Use

    Lentiviral, Mouse Targeting, and Synthetic Biolog…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 PARL-FLAG-CT S277G

    Plasmid

    #13615

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed catalytic Serine 277 to Alanine

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Gal4-PGC1 alpha

    Plasmid

    #8892

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Cell 1998 Mar 20;92(6):829-39.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Tags

    Gal4 DBD

    Expression

    Mammalian

    Available Since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pDN80

    Plasmid

    #61349

    Purpose

    LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Type

    Empty backbone

    Tags

    AsLOV2 wt and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL12009

    Plasmid

    #68257

    Purpose

    Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter and 5UTR Ubiquitin (Zea mays)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA/golgin-160/myc

    Plasmid

    #180632

    Purpose

    Expression of WT golgin-160

    Depositor

    Carolyn Machamer

    Article

    Hicks et al J Biol Chem. 2002 Sep 27. 277(39):35833-

    Insert

    golgin-160 (GOLGA3 Human)
    

    Tags

    Myc-His

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNSA-ARS-CRISPR-BlastR

    Plasmid

    #101009

    Purpose

    Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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