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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 21,271 results for: LEA

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Showing: 4941 - 4960 of 21271 results

  1. pYFP-NEAT1pr_v1-RT

    Plasmid
    #97087
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.
    Depositor
    Archa Fox
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nuclear paraspeckle assembly transcript 1 (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Promoter
    CMV
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97087-plasmid-map-sequence-id-189642

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  3. pDisplay-GRAB_OT1.0

    Plasmid
    #185384
    Purpose
    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells
    Depositor
    Yulong Li
    Publication
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based oxytocin sensor GRAB_OT1.0
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    185384-plasmid-map-sequence-id-366026

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  4. 2xFKBP-mCh-KDEL

    Plasmid
    #173010
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2xFKBP-mCh-KDEL
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173010-plasmid-map-sequence-id-339203

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  9. 5KO9 (HMCES)

    Plasmid
    #101877
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HMCES (HMCES Human)
    Tags
    6x His (modified C-term tag is AENLYFQSHHHHHH)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    101877-plasmid-map-sequence-id-194533

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  12. pOCC304

    Plasmid
    #118897
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with TEVprotease and TwinStrep-MB…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118897-plasmid-map-sequence-id-232369

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  13. pGBW-m4046768

    Plasmid
    #145763
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145763-plasmid-map-sequence-id-292506

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  15. pcDNA3-Akt-STOPS-NES

    Plasmid
    #175008
    Purpose
    Cytosol-targeted Akt-STOPS.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Akt-STOPS-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    175008-plasmid-map-sequence-id-344936

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  17. pX330-Flag-dSpCas9-HF1

    Plasmid
    #92115
    Purpose
    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9-HF1 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92115-plasmid-map-sequence-id-197505

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  20. YWHAG

    Plasmid
    #36903
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YWHAG (YWHAG Human)
    Tags
    6xHis with TEV Cleavage
    Expression
    Bacterial
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4941 - 4960 of 21271 results