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pSB700 lenti gRNA Blasto_zhang2.0

Plasmid

#167912

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA iRFP713

Plasmid

#167908

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-NGFR

Plasmid

#164520

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Publication

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Bxbi+PhiC31 GFP Bandpass BAC Reporter

Plasmid

#78218

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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Bxbi+TP901 GFP Bandpass BAC Reporter

Plasmid

#78229

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-YFP-HA no-pA

Plasmid

#82590

Purpose

3' entry vector containing HA epitope fused to C-terminus of YFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

YFP-HA (no pA)

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-HA no-pA

Plasmid

#82592

Purpose

3' entry vector with HA epitope fused to C-terminus of mCherry without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-HA (no pA)

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCas5

Plasmid

#82378

Purpose

sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCas61

Plasmid

#82397

Purpose

IPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpK

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

LDH

Mutation

V39R

Promoter

cLac143

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCas39

Plasmid

#82389

Purpose

sgRNA targeting glnA expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas36

Plasmid

#82388

Purpose

sgRNA targeting ccmK expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas35

Plasmid

#82387

Purpose

sgRNA targeting cpcB expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSLAX13 Flag (CT#231)

Plasmid

#14026

Depositor

Cliff Tabin

Publication

Logan et al Methods. 1998 Apr . 14(4):407-20.

Type

Empty backbone

Tags

Flag

Expression

Bacterial

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
TP.Rep2/Cap2-NF1A.EGFP

Plasmid

#260796

Purpose

Template plasmid expressing I-SceI site- and Ad5 ITR-flanked Rep2/Cap2-NF1A and AAV ITR-flanked EGFP (pHL492)

Depositor

Dan Wang

Publication

Liu et al Mol Ther. 2026 Aug 11:S1525-0016(26)0068

Insert

Rep2/Cap2-NF1A.EGFP

Use

AAV

Expression

Mammalian

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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TP.Rep2/Cap2-NF1A.GPR78

Plasmid

#260798

Purpose

Template plasmid expressing I-SceI site- and Ad5 ITR-flanked Rep2/Cap2-NF1A and AAV ITR-flanked GPR78 (pALD18)

Depositor

Dan Wang

Publication

Liu et al Mol Ther. 2026 Aug 11:S1525-0016(26)0068

Insert

Rep2/Cap2-NF1A.GPR78

Use

AAV

Expression

Mammalian

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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PGK-NbALFA-HaloTag

Plasmid

#253238

Purpose

Overexpress HaloTag-tagged NbALFA (ALFA-tag-binding nanobody) to enable live-cell imaging of ALFA-tagged proteins.

Depositor

May Malicdan

Insert

NbALFA

Use

Lentiviral

Tags

HaloTag

Promoter

PGK

Available since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pLCHKO-CRASP

Plasmid

#231928

Purpose

Empty backbone for CRASP-Seq experiments

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Behera et al Mol Cell. 2026 Jan 8;86(1):41-59.e15. do

Type

Empty backbone

Use

Lentiviral

Available since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBL066c

Plasmid

#227937

Purpose

Express Aβ42-GFP

Depositor

Tina Wang

Publication

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Aβ42

Tags

GFP (F64L, S65T)

Expression

Bacterial

Mutation

F64L and S65T in GFP

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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2015-N iFAST

Plasmid

#209415

Purpose

iFAST expression plasmid using MET3p

Depositor

Patrick Van Dijck

Publication

Devos et al Microbiology (Reading). 2024 Mar;170(3).

Insert

iFAST

Expression

Yeast

Promoter

MET3

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC31

Plasmid

#209996

Purpose

Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Publication

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

3' Connector (3C2SN)

Expression

Bacterial

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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