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We narrowed to 1,731 results for: plasmids pcas

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Showing: 481 - 500 of 1731 results

  2. pCAG-GluA1-scFV-Halo

    Plasmid
    #230065
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230065-plasmid-map-sequence-id-460116

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  5. pCAG-NLS-HA-Dre

    Plasmid
    #51272
    Purpose
    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    Dre
    Tags
    NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51272-plasmid-map-sequence-id-334322

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  10. pCAG-memRFP-3xControlgRNA

    Plasmid
    #224567
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224567-plasmid-map-sequence-id-451479

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  13. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

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  19. B-HypaR-SpCas9

    Plasmid
    #126764
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-Hypa2SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A; N692A; M694A; Q695A; H698A; amino acids 10…
    Promoter
    CBh
    Available Since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    126764-plasmid-map-sequence-id-410886

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  20. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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Showing: 481 - 500 of 1731 results