1. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pDONR223-DKFZp761P0423

   Plasmid

   #23475

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   DKFZp761P0423 (SGK223 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   GS inserted after aa343, del aa1068-1184

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

2. p3E_he1a:mCherry

   Plasmid

   #113881

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p3E_he1a:eYFP

   Plasmid

   #113879

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p3E_he1a:ECFP

   Plasmid

   #113880

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   ECFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-Enh eGFP Reporter-muKC2-INT2

   Plasmid

   #59287

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the keratin type II cluster.

   Depositor

   Benjamin Yu

   Insert

   krt80-Intron 4
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Mu Hsp68 minimal promoter

   Available since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pSP64-Vasa-GFP

   Plasmid

   #135595

   Purpose

   Expression of Vasa-GFP mRNA for microinjection for visualization of Vasa protein dynamics in S. purpuratus

   Depositor

   Mamiko Yajima

   Article

   Yajima et al Development. 2015 Jun 1;142(11):1960-70.

   Insert

   Vasa-GFP
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT2KXIGdeltaIn-MCS-birA-tagRFP

   Plasmid

   #58378

   Purpose

   tol2 transgenesis for biotin based TRAP - BirA ligase

   Depositor

   Didier Stainier

   Article

   Housley et al Development. 2014 Oct;141(20):3988-93. d

   Insert

   birA tagRFP
   

   Use

   Tags

   tagRFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT2KXIGdeltaIn-MCS-birA-EGFP

   Plasmid

   #58377

   Purpose

   tol2 transgenesis for biotin based TRAP - BirA ligase

   Depositor

   Didier Stainier

   Article

   Housley et al Development. 2014 Oct;141(20):3988-93. d

   Insert

   birA EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

   Plasmid

   #58380

   Purpose

   tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

   Depositor

   Didier Stainier

   Article

   Housley et al Development. 2014 Oct;141(20):3988-93. d

   Insert

   avi-EGFP-rpl10a
   

   Use

   Tags

   Avi Tag and EGFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-link-ymScarletI-URA3

    Plasmid

    #168055

    Purpose

    Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-link-ymTq2-URA3

    Plasmid

    #168057

    Purpose

    Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Use

    Tags

    ymTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-link-ymYPET-URA3

    Plasmid

    #168056

    Purpose

    Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymYPET
    

    Use

    Tags

    ymYPET

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-link-ytdTomato-URA3

    Plasmid

    #168054

    Purpose

    Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ytdTomato
    

    Use

    Tags

    ytdTomato

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3-ySet2

    Plasmid

    #51698

    Purpose

    Expresses full length yeast Set 2 gene in a mamalian expression vector

    Depositor

    Tom Misteli

    Article

    Luco et al Science. 2010 Feb 19;327(5968):996-1000.

    Insert

    yeast Set2 (SET2 Budding Yeast)
    

    Use

    Tags

    C-terminal FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pFA6a-link-ymVenus-URA3

    Plasmid

    #168058

    Purpose

    Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymVenus
    

    Use

    Tags

    ymVenus

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. plko_TRE3G_Myc-Sox9WT_PGKpuroR

    Plasmid

    #203962

    Purpose

    Dox inducible WT SOX9 with myc tag

    Depositor

    Elaine Fuchs

    Article

    Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

    Insert

    SOX9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-flex-rev-g2-2A-Venus

    Plasmid

    #71410

    Purpose

    This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.

    Depositor

    William Wisden

    Article

    Uygun et al J Neurosci. 2016 Nov 2;36(44):11171-1118

    Inserts

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse hdc gene promoter, works as a pan neuronal …

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. plko_TRE3G_Myc-Sox9HMGonlySTOP_PGKpuroR

    Plasmid

    #203963

    Purpose

    Dox inducible myc tag truncated SOX9 without trans-activating domain (noTA)

    Depositor

    Elaine Fuchs

    Article

    Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

    Insert

    SOX9-noTA (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mutation

    the TA domain is truncated

    Promoter

    TRE3G

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR

    Plasmid

    #203964

    Purpose

    Dox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)

    Depositor

    Elaine Fuchs

    Article

    Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

    Insert

    SOX9-noHMG (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mutation

    the HMG domain is truncated

    Promoter

    TRE3G

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-VSFP2.3 rev. with Flex

    Plasmid

    #87948

    Purpose

    Plasmid used (after linearization) to generate transgenic mice that express the genetically encoded voltage indicator upon Cre recombination.

    Depositor

    Thomas Knopfel

    Article

    Quinn et al Proc Natl Acad Sci U S A. 2016 Dec 20;11

    Insert

    Voltage-Sensitive Fluorescent Protein 2.3
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge