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pAAV-HDR-sphys-3XHA
Plasmid#163690Purposeplasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin geneDepositorInserttriple HA tag insertion into endogenous mouse synaptophysin gene
UseAAVTagstriple HA tagExpressionMutationPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-7D12Gln13TAG
Plasmid#125264PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertGln13TAG 7D12
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X dAmp/Kana+
Plasmid#154352PurposeEmpty backbone vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B IRES GFP
Plasmid#133817PurposeEncodes the C2B domain of synaptotagmin 1 for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12CTermTAG
Plasmid#125267PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertCTermTAG
UseTagsHis-TagExpressionMutationPromoterTrcAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Gly42TAG
Plasmid#125265PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Gly42 nanobody
UseTagsHis-tagExpressionBacterialMutationPromotertrc promoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMEH295 NLS dTomato 1x wild type MS2 loop
Plasmid#168214PurposeMammalian expression of nuclear-localized dTomato with 1 wild type MS2 loopDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X dAmp/Kana+-MT
Plasmid#154354PurposeBacterial expression of MTDepositorInsertMT
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X dAmp/Kana+-MTn
Plasmid#154355PurposeBacterial expression of MTnDepositorInsertMTn
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X dAmp/Kana+-MTbeta
Plasmid#154357PurposeBacterial expression of MTbetaDepositorInsertMTbeta
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X dAmp/Kana+-MTalpha
Plasmid#154356PurposeBacterial expression of MTalphaDepositorInsertMTalpha
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-SELF
Plasmid#158770PurposeExpression of synaptophysin 1 variant with self-cleaving c-terminal tail.DepositorInsertSynaptophysin 1-NS3/4a (Syt1 Mouse)
UseLentiviralTagsExpressionMutationFlexible linkers, ADVVCC/QMSY cleavage sitePromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP571
Plasmid#139498PurposeCRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNADepositorInsertpGPD Cas9 / sgRNA (STL162)
UseTagsExpressionYeastMutationWTPromoterpGPDAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A IRES GFP
Plasmid#133818PurposeEncodes the C2A domain of synaptotagmin 1 for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-01*(ΔL1)
Plasmid#80042PurposeExpression of PTAP L domain mutant EPN-01*DepositorInsertpCMV-EPN-01*(ΔL2)
UseTagsMycExpressionMammalianMutationPTAP sequence in p6 mutated to AlaPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 6: AIRAP(1-89).GST,V1.pcDNA3
Plasmid#21796DepositorInsertAIRAP (Zfand2a Mouse)
UseTagsGSTExpressionMammalianMutationAA 1-89PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 3: m1.9.9E10.pcDNA3
Plasmid#21793DepositorInsertAIRAP (Zfand2a Mouse)
UseTagsmycExpressionMammalianMutationlacks 5'UTRPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCherry-GFP-SNX9
Plasmid#170551PurposeTo monitor the turnover of SNX9 in lysosomesDepositorInsertSNX9 (SNX9 Human)
UseRetroviralTagsSNX9 in tandem with mCherry and GFPExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Antares2-N1
Plasmid#120869PurposePiggybac transposon plasmid for live animal optical imagingDepositorInsertAkaluc
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only
