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pPB-cT3G-cERH-ZNF281

Plasmid

#222923

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZNF281 (ZNF281 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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Sema6c-AP-His

Plasmid

#72041

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6d.4-AP-His

Plasmid

#72044

Purpose

Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.4 (Sema6d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3c(L)-AP-His

Plasmid

#72015

Purpose

Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4

Plasmid

#125148

Purpose

Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Insert

AcrIIA4-P2A-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pHDR2_DAZL-T2A-mGreenLantern-PuroTK

Plasmid

#222917

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-hcMYC (Plath)

Plasmid

#17966

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

v-myc myelocytomatosis viral oncogene homolog (avian) (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

n/a

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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pBabe(puro) mouse Egr3

Plasmid

#108102

Purpose

Retroviral vector for expression of mouse Egr3

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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Plxnb1(L)-Fc-His

Plasmid

#72126

Purpose

Expresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb1 (Plxnb1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3e(L)-AP-His

Plasmid

#72019

Purpose

Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3e (Sema3e Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-hKLF4 (Plath)

Plasmid

#17967

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

Kruppel-like factor 4 (gut) (KLF4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

N/A

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Sema4g-Fc-His

Plasmid

#72160

Purpose

Expresses the extracellular region of the Sema4G protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4g (Sema4g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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PVT1-luciferase

Plasmid

#113350

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Luciferase

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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Sema3g.a(L)-Fc-His

Plasmid

#72151

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2 (Plath)

Plasmid

#17965

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

SRY (sex determining region Y)-box 2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

N/A

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Plxna4-AP-His

Plasmid

#71999

Purpose

Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna4 (Plxna4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMP71-mIL15-IL12rb1-sushi-il12rb2

Plasmid

#174606

Purpose

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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MO70-mJP-4

Plasmid

#79598

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

Insert

Junctophilin-4 (Jph4 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
Sema6c-Fc-His

Plasmid

#72167

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only