We narrowed to 4,854 results for: U6...

1. pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI

   Plasmid

   #201684

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1 (V106W)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

   Plasmid

   #233036

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   RfxCas13d-compatible gRNA expression cassette and hfCas13d
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

   Plasmid

   #92120

   Purpose

   Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   MS2-P65-HSF1 (HSF1 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

   Plasmid

   #212962

   Purpose

   Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_non-targeting spacer
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

   Plasmid

   #28289

   Purpose

   3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)

   Depositor

   Gus Frangou, Alex Chenchik

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189924

   Purpose

   AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e V106W
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SaCas9 D10A, TadA ABE8e V106W

   Promoter

   EFS

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

   Plasmid

   #212963

   Purpose

   Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_GFP targeting spacer
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold

   Plasmid

   #226921

   Purpose

   Encoding SauriABE8e and sgRNA cassette on a single AAV

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   TadA8e, nSauriCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

   Plasmid

   #233035

   Purpose

   To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

   Insert

   RfxCas13d-compatible gRNA expression cassette and CasRx
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKL038 Lenti U6 dCas12a gRNA Puro mCherry

    Plasmid

    #195546

    Purpose

    dCas12a gRNA expression backbone

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti U6- empty cassette_Direct repeat_puro_mcherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)

    Plasmid

    #214733

    Purpose

    Expresses mCherry protein in infected and cre positive cells

    Depositor

    Félix Leroy

    Article

    de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

    Insert

    anti-Crh shRNA (scrambled) / mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid

    #133794

    Purpose

    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    NmeCas9 and Nme2Cas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    sgRNA architecture from Amrani et al. Genome Biol…

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

    Plasmid

    #172436

    Purpose

    Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    Cre
    mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

    Plasmid

    #84248

    Purpose

    Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgTRE3G
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid

    #194715

    Purpose

    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sites

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)

    Plasmid

    #209198

    Purpose

    Mutagenesis of Th

    Depositor

    Larry Zweifel

    Article

    Yu et al Neuron. 2021 Apr 21;109(8):1365-1380.e5.

    Insert

    Th (Th Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

    Plasmid

    #83925

    Purpose

    Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    eGFP
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Expression

    Mutation

    Promoter

    hUbC

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

    Plasmid

    #84250

    Purpose

    Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sa sgTRE3G
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

    Plasmid

    #129706

    Purpose

    Express cre-dependent KASH-EGFP and Gmeb1 shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

    Plasmid

    #194925

    Purpose

    mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

    Depositor

    Hao Xu

    Article

    Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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