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Plasmid#211180PurposeMammalian expression of full-length human STRAP. C-terminal HiBiT tag.DepositorInsertSTRAP:M1-A350 (STRAP Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-tracrRNA-PMtU6-26
Plasmid#209191PurposeCloning of sgRNAs for expression in plantsDepositorInsertMtU6-26 SnRNA promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-tracrRNA-PMtU6-1
Plasmid#209190PurposeCloning of sgRNAs for expression in plantsDepositorInsertMtU6-1 SnRNA promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-TRAK1 [N389/9R]
Plasmid#206567PurposeMammalian Expression Plasmid of anti-TRAK1 (Human). Derived from hybridoma N389/9.DepositorInsertanti-TRAK1 (Homo sapiens) recombinant Mouse monoclonal antibody (TRAK1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRANS-Pho2-1/2-2-Csy4
Plasmid#161763PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
UseTagsExpressionPlantMutationPromoterCmYLCV PromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRANS-Pho2-1/2-2-tRNA
Plasmid#161762PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
UseTagsExpressionPlantMutationPromoterCmYLCV PromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1C-Fluc-DmTra2_X
Plasmid#147922PurposeInsect Expression of DmTra2-3UTRDepositorInsertDmTra2-3UTR (tra2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 CP ABEMax strategy 2
Plasmid#135358PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)DepositorInsertWT Tada-nSpyCas9 aa 1029-evolved Tada
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 CP ABEMax strategy 3
Plasmid#135359PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminusDepositorInsert1029 CP nSpyCas9 ABEMax
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Opie2-NeoR[TraF]
Plasmid#131617PurposeExpresses a functional NeoR only in females and dsRed in both genders.DepositorInsertNeoR[traF]
UseTagsExpressionInsectMutationPromoterOpie2Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mTRAF3ip3∆TM-CFP
Plasmid#125182PurposeExpresses mouse TRAF3IP3 ∆TM in mammalian cellsDepositorInsertTRAF3ip3 (Traf3ip3 Mouse)
UseTagsCFPExpressionMammalianMutationK87R and S359P- See depositor comments belowPromoterCMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNA[Tra].1026G
Plasmid#112689Purposeexpress gRNA targeting Tra under dU6-3 promoterDepositorInsertU6.3-gRNA[Tra] (tra Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral N
Plasmid#113009PurposeFor bacterial expression of MBP fusion of N terminal region of Drosophila TralDepositorInsertN terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationamino acids 1-355PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral C
Plasmid#113008PurposeFor bacterial expression of MBP fusion of C terminal region of Drosophila TralDepositorInsertC terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationamino acids 356-652PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-Clover-Muro
Plasmid#82334PurposeVector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertClover-T2AMuro
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMeo
Plasmid#82502PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.DepositorInserteGFP-IRESMeo
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
STRADA gRNA (BRDN0001145789)
Plasmid#76053Purpose3rd generation lentiviral gRNA plasmid targeting human STRADADepositorInsertSTRADA (STRADA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STRADA gRNA (BRDN0001148926)
Plasmid#76054Purpose3rd generation lentiviral gRNA plasmid targeting human STRADADepositorInsertSTRADA (STRADA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STRADA gRNA (BRDN0001145392)
Plasmid#76055Purpose3rd generation lentiviral gRNA plasmid targeting human STRADADepositorInsertSTRADA (STRADA Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only