We narrowed to 1,451 results for: transfection

1. pARv-RFP

   Plasmid

   #60021

   Purpose

   pARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.

   Depositor

   Radislav Sedláček

   Article

   Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA AAVS1

   Plasmid

   #128119

   Purpose

   Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA AAVS1 + GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV/U6

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA EMX1

   Plasmid

   #128121

   Purpose

   Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA EMX1 + GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV/U6

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBOB-HA-Gnaq

   Plasmid

   #236750

   Purpose

   Express HA N-terminal tagged mouse Gq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

   Depositor

   Zhao Zhang

   Article

   Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

   Insert

   Gnaq (Gnaq Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-TO-g0 (FLP-IN)

   Plasmid

   #236120

   Purpose

   Plasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfection

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   g0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter with two TetR binding sites

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pABE-dGFP

   Plasmid

   #226863

   Purpose

   Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by A111V mutation, that can be reactivated by active adenine base editing

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A111V

   Promoter

   CMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBE-dGFP

   Plasmid

   #226861

   Purpose

   Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by Y93H mutation, that can be reactivated by active cytosine base editing

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y93H

   Promoter

   CMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.23_Ptgs2

   Plasmid

   #209519

   Purpose

   Expresses luciferase under control of Ptgs2 promoter in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

   Insert

   Ptgs2 (Ptgs2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tol2-GgVCL/WT

   Plasmid

   #162787

   Purpose

   CONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

   Depositor

   Laura Lafon-Hughes

   Article

   et al PeerJ

   Insert

   Gallus gallus vinculin (VCL Chicken)
   

   Use

   Tags

   IRES-GFP

   Expression

   Bacterial and Mammalian

   Mutation

   WT

   Promoter

   beta actin

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA HBB

    Plasmid

    #128120

    Purpose

    Co-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA HBB + GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV/U6

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA RUNX

    Plasmid

    #128122

    Purpose

    Co-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA RUNX + GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV/U6

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgRNA HEK

    Plasmid

    #128123

    Purpose

    Co-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA HEK + GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV/U6

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Thy1.1-muSOX D219A

    Plasmid

    #131704

    Purpose

    encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    Thy1.1-muSOX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Aspartic acid 219 mutated to an alanine

    Promoter

    CMV

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xMma PylT sfGPF 150TAG

    Plasmid

    #154766

    Purpose

    plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, U25C in PylT

    Promoter

    EF1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-GFP

    Plasmid

    #137763

    Purpose

    Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GFP
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xMx PylT_GFP 150TAG

    Plasmid

    #140016

    Purpose

    Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG, K46E (Please see depositor comments below)

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. shh10

    Plasmid

    #64867

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

    Depositor

    David Schaffer, John Flannery

    Article

    Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

    Insert

    shh10 cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    I319V, N451D, D532N

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-GLUT5 in pRK5

    Plasmid

    #154205

    Purpose

    N-terminal HA-tagged GLUT5 cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    SLC2A5 (SLC2A5 Human)
    

    Use

    Tags

    N terminal HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Flag-NPM1wt

    Plasmid

    #131810

    Purpose

    Expresses Flag-NPM1wildtype (human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOO2-GW

    Plasmid

    #135097

    Purpose

    Gateway vector for expression of proteins in Xenopus oocytes

    Depositor

    Wolf Frommer

    Article

    Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

    Type

    Empty backbone

    Use

    Bacterial expression vector for generating sp6 tr…

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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